Interleukin-10 in serous ovarian carcinoma cell lines
Interleukin-10 in serous ovarian carcinoma cell lines
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DOI：
10.1007/s002620100196
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发表时间：
2001-08-01
影响因子：
5.8
通讯作者：
Hauptmann, S
中科院分区：
文献类型：
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作者：
Berger, S;Siegert, A;Hauptmann, S
白介素10是最有效的抗炎细胞因子之一，体内表达于卵巢癌中。与腹水和肿瘤组织中高水平的IL-10相比，这种细胞因子的表达在体外卵巢癌细胞系中似乎是罕见的。对卵巢癌细胞系中IL-10表达的调控几乎一无所知。我们研究了来源于卵巢浆液性腺癌的四种细胞系：OVCAR-3、SKOV-3、CAOV-3和OAW-42中IL-10的表达。在OVCAR-3细胞中检测到IL-10特异性的mRNA，并且只有该细胞系在无血清和有血清条件下都能组成性地产生IL-10。我们对OVCAR-3分泌IL-10的调节研究表明：(1)促炎刺激IL-1β和TNF-α促进IL-10的分泌，而不是内毒素，(2)IL-6对IL-10的分泌没有影响，(3)前列腺素E_2既不影响自发产生的IL-10，也不影响由TNF-α或IL-1β刺激的IL-10的产生，(4)干扰素-γ抑制IL-10的分泌。我们得出结论，只有少数浆液性卵巢癌细胞在体外保持产生IL-10的能力。我们在OVCAR-3中对IL-10产生的调节数据表明，卵巢癌细胞具有典型的单核细胞分泌IL-10的典型调节特征，但不是全部。
Interleukin-10, one of the most potent anti-inflammatory cytokines, is expressed in ovarian carcinomas in vivo. In contrast to the high levels of IL-10 in ascites and tumour tissue, the expression of this cytokine appears to be a rare event in ovarian carcinoma cell lines in vitro. Virtually nothing is known about the regulation of IL-10 expression in ovarian carcinoma cell lines. We investigated the expression of IL-10 in four cell lines originally derived from ovarian serous adenocarcinoma: OVCAR-3, SKOV-3, CAOV-3 and OAW-42. IL-10-specific mRNA was detected in OVCAR-3 and only this cell line produced IL-10 constitutively under serum-free conditions as well as in serum-containing medium. Our studies on the regulation of IL-10 secretion in OVCAR-3 revealed that (1) proinflammatory stimuli IL-1 beta and TNF-alpha, but not LPS, enhance IL-10 secretion, (2) IL-6 has no influence on the release of IL-10, (3) prostaglandin E2 influences neither the spontaneous nor the TNF-alpha- or IL-1 beta -stimulated IL-10 production and (4) interferon-gamma inhibits IL-10 secretion. We conclude that only a minority of serous ovarian carcinoma cells maintain the ability to produce IL-10 in vitro. Our data on the regulation of IL-10 production in OVCAR-3 indicate that ovarian carcinoma cells share some, but not all, of the regulatory features typical for the monocytic IL-10 secretion.