COMPARATIVE STUDIES OF THE AGE-RELATED-CHANGES IN PROTEIN-SYNTHESIS IN THE RAT PANCREAS AND PAROTID-GLAND
COMPARATIVE STUDIES OF THE AGE-RELATED-CHANGES IN PROTEIN-SYNTHESIS IN THE RAT PANCREAS AND PAROTID-GLAND
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DOI：
10.1016/0531-5565(81)90012-7
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发表时间：
1981-01-01
影响因子：
3.9
通讯作者：
HARTOG, VW
中科院分区：
文献类型：
--
作者：
KIM, SK;WEINHOLD, PA;HARTOG, VW
研究大鼠胰腺蛋白质合成是否随年龄变化，并与腮腺唾液腺的变化模式进行比较。在两个腺体中，3h -亮氨酸并入酸不溶性蛋白的速率随年龄的增长而下降。在胰腺，掺入率在12个月时达到峰值，在18个月、24个月和30个月时分别下降了21.4%和64%。在腮腺中，氨基酸掺入在2月龄时达到最高水平，而在12、18、24和30月龄时，氨基酸掺入量分别下降了2月龄时的21.9%、29.9%、49%和58%。在3 - h -亮氨酸并入两个腺体的酸溶性部分的速率没有年龄相关的差异。细胞水平。老龄大鼠（24岁和30岁）胰腺和腮腺-淀粉酶减少。细胞内α水平的差异。-淀粉酶活性与胰腺腺泡细胞分泌颗粒数量的差异相关，说明淀粉酶水平反映了细胞分泌蛋白的含量。在年轻和年老大鼠的这些腺体中，没有可检测到的形态变化与蛋白质合成减少50%相对应。
Whether changes occur in protein synthesis with age in the pancreas of the rat was investigated, and the pattern of changes was compared with that observed in parotid salivary glands. The rate of incorporation of 3H-leucine into acid-insoluble proteins declined with age in both glands. In the pancreas, the rate of incorporation peaked at 12 mo. and declined by 21, 54 and 64% of this level at 18, 24 and 30 mo., respectively. In parotid glands, the highest level of the amino acid incorporation occurred at 2 mo. and the level declined by 21, 29, 49 and 58% of the 2 mo. level at 12, 18, 24 and 30 mo., respectively. There was no age-related difference in the rate of incorporation of 3H-leucine into acid-soluble fractions of the 2 glands. The cellular level of .alpha.-amylase was reduced in the pancreas and parotid gland of old (24 and 30 mo. old) rats. The differences in the cellular level of .alpha.-amylase activity among the age groups correlated with differences in the number of secretory granules present in the acinar cells of the pancreas, indicating that the amylase level reflected the cellular content of secretory proteins. There was no detectable morphological change that paralleled the > 50% reduction in protein synthesis in these glands of young and old rats.