Preserved Function of Circulating Invariant Natural Killer T Cells in Patients With Chronic Hepatitis B Virus Infection.
Preserved Function of Circulating Invariant Natural Killer T Cells in Patients With Chronic Hepatitis B Virus Infection.
复制标题
慢性乙型肝炎病毒感染患者循环不变自然杀伤 T 细胞的功能保留。
DOI：
10.1097/md.0000000000000961
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发表时间：
2015-06
期刊：
影响因子：
1.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Zhu H;Zhang Y;Liu H;Zhang Y;Kang Y;Mao R;Yang F;Zhou D;Zhang J
补充数字内容可在文本中获得到目前为止，不变的自然杀伤T（iNKT）细胞在慢性B型肝炎病毒（HBV）感染中的作用尚未完全了解。在以前的报道中，iNKT细胞通过间接方法鉴定。然而，观察到HBV感染期间iNKT细胞的流行率和功能存在差异。在这项研究中，我们设计了一种直接的，高度特异性的CD 1d四聚体为基础的方法来测试是否与HBV感染的患者有相关的iNKT细胞缺陷。在我们的研究中，共有93名慢性HBV感染者和30名健康人（作为对照）参加。iNKT细胞的流行，他们的细胞因子产生能力，并在体外扩增通过流式细胞术分析与CD 1d四聚体染色。我们的观察结果表明，HBV感染患者和健康对照组之间的循环CD 1d-四聚体阳性iNKT细胞数量无显著差异。iNKT细胞产生IFN-γ或IL-4的能力以及它们的体外扩增在这两组之间也是相当的。然而，在慢性HBV感染患者中，与免疫耐受（IT）患者相比，在慢性B肝炎（CHB）和肝硬化患者中观察到iNKT细胞数量减少。这些结果表明，慢性HBV感染患者可能具有正常的流行率和保留的循环iNKT细胞功能。核苷类似物抗病毒治疗并不改变慢性B型肝炎患者循环iNKT细胞的频率和功能。
Supplemental Digital Content is available in the text To date, the role of invariant natural killer T (iNKT) cells in chronic hepatitis B virus (HBV) infection is not fully understood. In previous reports, iNKT cells were identified by indirect methods. However, discrepancies regarding the prevalence and function of iNKT cells during HBV infection were observed. In this study, we have devised a direct, highly specific CD1d tetramer-based methodology to test whether patients with HBV infection have associated iNKT-cell defects. In our study, a total of 93 chronic HBV-infected patients and 30 healthy individuals (as control) were enrolled. The prevalence of iNKT cells, their cytokine producing capacity, and in vitro expansion were determined by flow cytometric analysis with CD1d tetramer staining. Our observation demonstrated that there was no significant difference in circulating CD1d-tetramer positive iNKT cell numbers between HBV-infected patients and healthy controls. The capacity of iNKT cells to produce IFN-γ or IL-4 as well as their in vitro expansion was also comparable between these 2 groups. However, among chronic HBV-infected patients, a decrease in iNKT cell-number was observed in chronic hepatitis B (CHB) and cirrhosis patients in comparison to that in immune tolerant (IT) patients. These results indicated that patients with chronic HBV infection may have normal prevalence and preserved function of circulating iNKT cells. And antiviral therapy with nucleot(s)ide analogue does not alter the frequency and function of circulating iNKT cells in chronic Hepatitis B patients.