Prognostic value of FoxP3 and CTLA-4 expression in patients with oral squamous cell carcinoma
Prognostic value of FoxP3 and CTLA-4 expression in patients with oral squamous cell carcinoma
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DOI：
10.1371/journal.pone.0237465
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发表时间：
2020-08-12
期刊：
影响因子：
3.7
通讯作者：
Miyazaki, Akihiro
中科院分区：
文献类型：
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作者：
Koike, Kazushige;Dehari, Hironari;Miyazaki, Akihiro
背景肿瘤浸润淋巴细胞包括肿瘤反应性淋巴细胞和调节性T细胞。然而，肿瘤浸润淋巴细胞在口腔鳞状细胞癌（OSCC）中的预后价值仍不清楚。方法我们使用免疫组织化学方法评估肿瘤浸润FoxP 3（+）T细胞和CTLA-4（+）细胞在四个不同组织学分区中的存在（肿瘤中心的肿瘤实质和基质，以及浸润前沿的实质和间质），并评估这些细胞的流行率与137例OSCC患者的组织病理学状态之间的关系。高FoxP（+）患者的疾病特异性生存率和无复发生存率更高。浸润前沿实质中的T细胞。结论浸润前沿实质中FoxP 3（+）T细胞的存在可能是一个有用的预后因子。我们的研究结果表明，FoxP 3（+）T细胞可能发挥位点特异性抗肿瘤作用，但可能不发挥免疫抑制作用，在口腔鳞癌。此外，我们的研究结果表明，CTLA-4（+）细胞抑制FoxP 3（+）T细胞的功能，并促进抗肿瘤免疫在口腔鳞癌。
Background Tumor-infiltrating lymphocytes include tumor-reactive lymphocytes and regulatory T-cells. However, the prognostic value of tumor-infiltrating lymphocytes in oral squamous cell carcinoma (OSCC) remains unclear.Methods We used immunohistochemistry to evaluate the presence of tumor-infiltrating FoxP3(+) T-cells and CTLA-4(+) cells in four distinct histological compartments (tumor parenchyma and stroma at the tumor center, and parenchyma and stroma at the invasive front) and assessed the association between the prevalence of these cells and the histopathological status of 137 patients with OSCC.Results Five-year overall survival, disease-specific survival, and recurrence-free survival were favorable in patients with high numbers of FoxP(+). T-cells in the parenchyma of the invasive front. Recurrence-free survival and metastasis-free survival were decreased in patients with high numbers of CTLA-4(+) cells in the parenchyma of the invasive front.Conclusions The presence of FoxP3(+) T-cells in the parenchyma of the invasive front may be a useful prognostic factor. Our results indicate that FoxP3(+) T-cells may exert site-specific anti-tumor effects but may not play an immunosuppressive role in OSCC. In addition, our results suggest that CTLA-4(+) cells suppress the function of FoxP3(+) T-cells and promote anti-tumor immunity in OSCC.