A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity
A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunity
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DOI：
10.1126/science.1073759
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发表时间：
2002-07-26
期刊：
影响因子：
56.9
通讯作者：
Ausubel, FM
中科院分区：
文献类型：
--
作者：
Kim, DH;Feinbaum, R;Ausubel, FM
对对铜绿假单胞菌致死敏感性增强的秀丽线虫突变体进行遗传筛选，鉴定出两个抗病原体所需的基因：编码丝裂原激活蛋白(MAP)激酶的sek-1和编码MAP激酶的NSY-1。RNA干扰分析和生化分析证实，p38同源基因PMK-1是病原菌防御所需的下游MAP激酶。这些数据表明，这个MAP激酶信号盒代表了进化不同物种中先天免疫反应的一个古老特征。
A genetic screen for Caenorhabditis elegans mutants with enhanced susceptibility to killing by Pseudomonas aeruginosa led to the identification of two genes required for pathogen resistance: sek-1, which encodes a mitogen-activated protein (MAP) kinase kinase, and nsy-1, which encodes a MAP kinase kinase kinase. RNA interference assays and biochemical analysis established that a p38 ortholog, pmk-1, functions as the downstream MAP kinase required for pathogen defense. These data suggest that this MAP kinase signaling cassette represents an ancient feature of innate immune responses in evolutionarily diverse species.