High-Throughput, Accurate Mass Metabolome Profiling of Cellular Extracts by Flow Injection - Time-of-Flight Mass Spectrometry
High-Throughput, Accurate Mass Metabolome Profiling of Cellular Extracts by Flow Injection - Time-of-Flight Mass Spectrometry
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DOI：
10.1021/ac201267k
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发表时间：
2011-09-15
影响因子：
7.4
通讯作者：
Zamboni, Nicola
中科院分区：
文献类型：
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作者：
Fuhrer, Tobias;Heer, Dominik;Zamboni, Nicola
在高分辨率质谱仪上直接进样样品是一种有效的方法，可以最大限度地提高分析通量，同时还可以通过质荷比在复杂样品中区分分析物。我们提出了一个流动注射电喷雾-飞行时间质谱的平台，以自然质量分辨率对每天> 1400个生物提取物中的小分子进行分析。我们通过5000多次注射化学定义的标准品和从微型培养中获得的大肠杆菌细胞提取物来全面衡量性能。对于至少90%的测试化合物，我们在30年的浓度范围内获得线性响应，日间变异系数为
Direct injection of samples on high-resolving mass spectrometers is an effective way to maximize analytical throughput and yet allow analyte discrimination in complex samples by mass-to-charge ratio. We present a platform of flow injection electrospray-time-of-flight mass spectrometry to profile small molecules in > 1400 biological extracts per day at native mass resolution. We comprehensively benchmark the performance with more than 5000 injections of chemically defined standards and Escherichia coli cellular extracts obtained from miniscale cultivations. For at least 90% of tested compounds, we attain a linear response over 3 decades of concentration, interday coefficient of variation of