An evaporation-based microfluidic sample concentration method
An evaporation-based microfluidic sample concentration method
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DOI：
10.1039/b202473j
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发表时间：
2002-01-01
期刊：
影响因子：
6.1
通讯作者：
Beebe, DJ
中科院分区：
文献类型：
--
作者：
Walker, GM;Beebe, DJ
我们提出了一种利用蒸发诱导流在微流控装置内进行样品浓缩的方法。蒸发诱导流很容易被纳入微流控设计，可以用来浓缩各种各样的分子。用0.2 μ m荧光球和FITC标记的牛血清白蛋白验证了该方法的实用性。32%的0.6 μ l荧光球悬浮液被浓缩到微流控装置内的孔中。在相同的时间内，93%的0.6 μ l FITC标记的BSA溶液被浓缩。
We present a method for sample concentration within microfluidic devices using evaporation-induced flow. Evaporation-induced flow is easy to incorporate into microfluidic designs and can be used to concentrate a wide variety of molecules. The practicality of this method was demonstrated with 0.2 mum fluorescent spheres and FITC- labeled BSA. Thirty two percent of the 0.6 muL fluorescent sphere suspension was concentrated into a well within a microfluidic device. In the same amount of time, 93% of the 0.6 muL FITC- labeled BSA solution was concentrated.