A single nucleotide change in a core promoter is involved in the progressive overexpression of the duplicated CYP9M10 haplotype lineage in Culex quinquefasciatus
A single nucleotide change in a core promoter is involved in the progressive overexpression of the duplicated CYP9M10 haplotype lineage in Culex quinquefasciatus
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DOI：
10.1016/j.ibmb.2015.10.006
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发表时间：
2015-11-01
影响因子：
3.8
通讯作者：
Tomita, Takashi
中科院分区：
文献类型：
--
作者：
Itokawa, Kentaro;Komagata, Osamu;Tomita, Takashi
虽然解毒酶基因上的顺式作用突变对杀虫剂抗性的重要性被广泛接受，但迄今为止只有少数突变被确定为基因组DNA中存在的具体突变。致倦库蚊细胞色素P450基因CYP 9 M10的过表达与拟除虫菊酯抗性相关表现出过表达的CYP 9 M10单倍型（耐药单倍型）属于一个特定的系统发育谱系，该谱系具有高核苷酸序列同源性和相同的转座因子插入。在耐药单倍型中，涉及额外顺式作用突变和基因重复的等位基因进展进化出与极高转录和强拟除虫菊酯耐药性相关的CYP 9 M10单倍型。在这里，我们表明，一个单核苷酸取代G-27 A，这是位于附近的转录起始位点的CYP 9 M10，参与了重复的单倍型谱系的进展。一个7-bp的AT-丰富的序列，包括核苷酸-27的删除抑制从原来的转录起始位点的转录起始。该突变被怀疑位于CYP 9 M10的核心启动子TATA盒内。(C)2015作者爱思唯尔有限公司出版
Although the importance of cis-acting mutations on detoxification enzyme genes for insecticide resistance is widely accepted, only a few of them have been determined as concrete mutations present in genomic DNA till date. The overexpression of a cytochrome P450 gene, CYP9M10, is associated with pyrethroid resistance in the southern house mosquito Culex quinquefasciatus. The haplotypes of CYP9M10 exhibiting overexpression (resistant haplotypes) belong to one specific phylogenetic lineage that shares high nucleotide sequence homology and the same insertion of a transposable element. Among the resistant haplotypes, allelic progression involving an additional cis-acting mutation and gene duplication evolved a CYP9M10 haplotype associated with extremely high transcription and strong pyrethroid resistance. Here we show that a single nucleotide substitution G-27A, which is located near the transcription start site of CYP9M10, is involved in the progression of the duplicated haplotype lineage. The deletion of a 7-bp AT-rich sequence that includes nucleotide -27 inhibited the initiation of transcription from the original transcriptional initiation site. The mutation was suspected to reside within a core promoter, TATA-box, of CYP9M10. (C) 2015 The Authors. Published by Elsevier Ltd.