Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina
Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina
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DOI：
10.1038/s41598-019-45750-7
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发表时间：
2019-06-27
影响因子：
4.6
通讯作者：
Emerson, Mark M.
中科院分区：
文献类型：
--
作者：
Schick, Estie;McCaffery, Sean D.;Emerson, Mark M.
在脊椎动物视网膜发育过程中，形成了具有有限细胞命运选择的短暂视网膜祖细胞群。这些祖细胞群之一表达 Thrb 基因，可通过 ThrbCRM1 顺式调控元件的活性进行识别。短期测定得出的结论是，这些细胞优先产生视锥光感受器和水平细胞，然而发育时间阻碍了对其后代的广泛细胞类型表征。在这里，我们描述了基于重组酶的鸡胚谱系追踪系统的开发和验证，以进一步表征这些细胞的谱系。人们发现ThrbCRM1元件优先形成光感受器和水平细胞，以及少量的视网膜神经节细胞。光感受器细胞后代完全是视锥细胞光感受器，而不是视杆细胞光感受器，这证实了ThrbCRM1祖细胞受到视杆细胞命运的限制。此外，水平细胞和视网膜神经节细胞的特定亚型过多，表明ThrbCRM1祖细胞不仅受到细胞类型的限制，而且还受到细胞亚型的限制。
During vertebrate retinal development, transient populations of retinal progenitor cells with restricted cell fate choices are formed. One of these progenitor populations expresses the Thrb gene and can be identified by activity of the ThrbCRM1 cis-regulatory element. Short-term assays have concluded that these cells preferentially generate cone photoreceptors and horizontal cells, however developmental timing has precluded an extensive cell type characterization of their progeny. Here we describe the development and validation of a recombinase-based lineage tracing system for the chicken embryo to further characterize the lineage of these cells. The ThrbCRM1 element was found to preferentially form photoreceptors and horizontal cells, as well as a small number of retinal ganglion cells. The photoreceptor cell progeny are exclusively cone photoreceptors and not rod photoreceptors, confirming that ThrbCRM1 progenitor cells are restricted from the rod fate. In addition, specific subtypes of horizontal cells and retinal ganglion cells were overrepresented, suggesting that ThrbCRM1 progenitor cells are not only restricted for cell type, but for cell subtype as well.