Cytogenetic stability of maize tissue cultures: a cell line pedigree analysis
Cytogenetic stability of maize tissue cultures: a cell line pedigree analysis
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DOI：
10.1139/g88-056
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发表时间：
1988-06
期刊：
影响因子：
3.1
通讯作者：
G. Benzion;R. Phillips
中科院分区：
文献类型：
--
作者：
G. Benzion;R. Phillips
Cytogenetic instability in maize plants regenerated from tissue culture is a commonly observed phenomenon. In an attempt to understand the origin of this instability meiotic analyses were performed on 370 regenerated plants that were initiated from cell lines of 22 immature embryos of 9 maize genotypes. Cell lineage pedigrees were maintained on these cultures to record the familial relationship between regenerated plants. Overall, 12.4% of the 370 regenerants contained cytological aberrations. The largest category of aberrations involved chromosome breakage (translocations, deletions, and an inversion) between a heterochromatic knob or knob site and the centromere. Late replicating knob heterochromatin might have been responsible for the chromosome breakage events. Cytologically identical aberrations were observed in plants regenerated at various time points in the pedigreed cultures, indicating an early occurrence of the aberration during culture maintenance. Heterogeneity of cell karyotypes also was obs...
