Genetic distance between Nasutitermes takasagoensis (Isoptera: Termitidae) populations in Taiwan and the Yaeyama Islands, analyzed by amplified fragment length polymorphism markers
Genetic distance between Nasutitermes takasagoensis (Isoptera: Termitidae) populations in Taiwan and the Yaeyama Islands, analyzed by amplified fragment length polymorphism markers
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DOI：
10.1111/j.1479-8298.2004.00070.x
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发表时间：
2004-09
影响因子：
0.9
通讯作者：
Julio García;K. Maekawa;T. Miura;Tadao Matsumoto
中科院分区：
文献类型：
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作者：
Julio García;K. Maekawa;T. Miura;Tadao Matsumoto
种群的扩散能力和近交程度可以通过遗传标记间接评估。有人认为，有翼白蚁的飞行距离不能超过几百米。利用扩增片段长度多态性（AFLP）技术，对日本南部八山群岛的Nasutitermes takasagoensis种群亚结构进行了分析。基于AFLP数据，我们发现存在适度的亚种群结构。此外，相对较低的Gst和岛屿种群之间的遗传距离表明，有翼白蚁有可能能够飞行几公里的距离。为了进一步了解这一点，在本研究中，我们比较了在台湾南部衡川半岛采集的新样本与先前在八山群岛采集的样本。利用在台湾收集的样本，计算遗传距离与六个八山岛的样本。计算遗传多样性、平均杂合度、多态位点百分率、Gst和基因流量（Nm）。3个引物组合共获得155个AFLP片段，多态性条带8条（5%）。台湾种群与其他岛屿之间的遗传距离大于八山群岛之间的遗传距离。虽然以往的研究表明八山列岛的平均Nm为2.47，但台湾与八山列岛之间的观测Nm均小于1。这些发现表明台湾与八山群岛之间的地理距离足够大，可以阻止它们之间的基因流动。
Dispersal ability and degree of inbreeding in a population can indirectly be assessed using genetic markers. It has been suggested that winged termites are not able to fly distances greater than several hundred meters. Using the amplified fragment length polymorphism (AFLP) technique, we previously analyzed the population substructure of Nasutitermes takasagoensis in the Yaeyama Islands, southern Japan. We found that there is moderate subpopulation structure based on the AFLP data. Moreover, the relatively low Gst and genetic distances among insular populations suggest that there is a possibility that winged termites are able to fly over distances of several kilometers. To learn more about this, in the present study, we compared new samples collected from the Hengchuen Peninsula in southern Taiwan with samples previously collected from the Yaeyama Islands. Using the samples collected in Taiwan, genetic distance was calculated against samples from six of the Yaeyama Islands. Genetic diversity, average heterozygosity, percentage of polymorphic loci, Gst and gene flow (Nm) were calculated. A total of 155 AFLP fragments were obtained for three primer combinations used, with 8 (5%) polymorphic bands. Genetic distance between the populations of Taiwan and the rest of the islands was greater than the genetic distance among the Yaeyama Islands. Although previous studies showed that average Nm among the Yaeyama Islands was 2.47, all of the observed Nm between Taiwan and the Yaeyama Islands were below 1. These findings suggest that the geographic distance between Taiwan and the Yaeyama Islands is large enough to prevent gene flow between them.