Quantitative evaluation of marine protein contribution in ancient diets based on nitrogen isotope ratios of individual amino acids in bone collagen: an investigation at the Kitakogane Jomon site.
Quantitative evaluation of marine protein contribution in ancient diets based on nitrogen isotope ratios of individual amino acids in bone collagen: an investigation at the Kitakogane Jomon site.
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DOI：
10.1002/ajpa.21287
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发表时间：
2010-09
影响因子：
2.8
通讯作者：
Y. Naito;Noah V. Honch;Y. Chikaraishi;N. Ohkouchi;M. Yoneda
中科院分区：
文献类型：
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作者：
Y. Naito;Noah V. Honch;Y. Chikaraishi;N. Ohkouchi;M. Yoneda
骨胶原蛋白氨基酸的氮稳定同位素分析作为一种新的工具，用于评估古代人类的饮食组成和校准的放射性碳年代考古样品。我们使用这种技术来调查日本北海道的Kitakogane贝壳贝冢（5，300 - 6，000 cal BP）的人类和动物样本。使用单个氨基酸的化合物特定氮同位素分析，我们的目的是估计i）海洋和陆地蛋白质对人类饮食的定量贡献，以及ii）海洋生态系统膳食蛋白质来源的平均营养水平（TL）。参照氨基酸营养级（TL（AA））模型解释数据，该模型使用现代海洋动物的经验氨基酸δ（15）N构建预测生物体营养位置的数学方程。TL（AA）模型产生了现实的TL估计北古钱海洋动物。然而，这个模型不适合解释人类氨基酸δ（15）N，因为膳食蛋白质来自海洋和陆地环境。因此，我们开发了一系列相关的方程，考虑了两个生态系统的饮食资源的消耗。使用这些公式，估计Kitakogane人类饮食中海洋蛋白质的平均百分比为74%。虽然这项研究是考古骨胶原中氨基酸δ（15）N的首次系统研究之一，但我们相信这项技术对于TL重建，古饮食解释以及校正放射性碳测年的海洋储层影响非常有用。
Nitrogen stable isotopes analysis of individual bone collagen amino acids was applied to archeological samples as a new tool for assessing the composition of ancient human diets and calibrating radiocarbon dates. We used this technique to investigate human and faunal samples from the Kitakogane shell midden in Hokkaido, Japan (5,300-6,000 cal BP). Using compound-specific nitrogen isotope analysis of individual amino acids, we aimed to estimate i) the quantitative contribution of marine and terrestrial protein to the human diet, and ii) the mean trophic level (TL) from which dietary protein was derived from marine ecosystems. Data were interpreted with reference to the amino acid trophic level (TL(AA)) model, which uses empirical amino acid delta(15)N from modern marine fauna to construct mathematical equations that predict the trophic position of organisms. The TL(AA) model produced realistic TL estimates for the Kitakogane marine animals. However, this model was not appropriate for the interpretation of human amino acid delta(15)N, as dietary protein is derived from both marine and terrestrial environments. Hence, we developed a series of relevant equations that considered the consumption of dietary resources from both ecosystems. Using these equations, the mean percentage of marine protein in the Kitakogane human diet was estimated to be 74%. Although this study is one of the first systematic investigations of amino acid delta(15)N in archeological bone collagen, we believe that this technique is extremely useful for TL reconstruction, palaeodietary interpretation, and the correction of marine reservoir effects for radiocarbon dating.