Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
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DOI：
10.1016/j.febslet.2005.05.022
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发表时间：
2005-07-04
期刊：
影响因子：
3.5
通讯作者：
Soares, H
中科院分区：
文献类型：
--
作者：
Nolasco, S;Bellido, J;Soares, H
微管是参与细胞基本功能的α/β微管蛋白聚合物。一个涉及不同分子伴侣和辅因子的多步骤过程产生了能够聚合的新的微管蛋白杂二聚体。在体外，辅因子A(TBCA)以分子伴侣的形式与β-微管蛋白以准天然状态相互作用。我们已经使用siRNA沉默了HeLa和MCF-7哺乳动物细胞系中TBCA的表达。TBCA对细胞活力至关重要，它的敲除会导致可溶性微管蛋白的减少、微管的修饰和G1期细胞周期的停滞。在MCF-7细胞中，细胞死亡之前是类似分化的细胞形状的变化。(C)2005年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Microtubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.