Temporal and spatial heterogeneity of mtDNA polymorphisms in natural populations of Drosophila mercatorum.
Temporal and spatial heterogeneity of mtDNA polymorphisms in natural populations of Drosophila mercatorum.
复制标题
果蝇自然群体线粒体DNA多态性的时空异质性。
DOI：
10.1093/genetics/116.2.215
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Johnston,JS
中科院分区：
文献类型：
--
作者：
DeSalle,R;Templeton,A;Mori,I;Pletscher,S;Johnston,JS
用限制性内切酶分析了mercatorum果蝇亚群几个地理上分离的等位基因的线粒体DNA的遗传关系。此外，我们还研究了由酶BstE Ⅱ和BstNI在一个单一的地点--夏威夷的Kamuela产生的两个mtDNA限制性位点多态性的时间和空间分布。由于小样本量的一些集合和不期望的依赖性的多态性频率的估计其方差，反正弦平方根转换的频率数据。我们还使用我们的转换频率的anFstestimation，以证明相当大的空间和时间内的Kamuela人口的分化。与此相反，从同一人口的同工酶数据显示没有分化模式。线粒体DNA分析的时间和地理异质性和种群细分也与该物种已知的扩散行为和生态限制相一致。线粒体DNA数据结合同工酶数据表明，KamuelaD. mercatorum是接近的分界线分开panmixia和细分，一个结论，不能单独从同工酶数据。
Restriction endonuclease analysis of mtDNA was used to examine the genetic relatedness of several geographically separated isolines of theDrosophila mercatorumsubgroup. In addition, we examined the temporal and spatial distribution of two mtDNA restriction site polymorphisms produced by the enzymesBstEII andBstNI at a single locality—Kamuela, Hawaii. Due to small sample sizes of some collections and the undesirable dependance of the estimation of polymorphism frequency on its variance, an arcsin square root transformation of the frequency data was used. We also use anFstestimator of our transformed frequencies to demonstrate considerable spatial and temporal differentiation within the Kamuela population. In contrast, isozyme data from the same population reveals no pattern of differentiation. The temporal and geographic heterogeneity and population subdivision detected with mtDNA analysis also is consistent with the known dispersal behavior and ecological constraints of this species. The mtDNA data in conjunction with the isozyme data show that the population structure of the KamuelaD. mercatorumis close to the boundary line separating panmixia from subdivision, a conclusion that could not be made from isozyme data alone.