INVOLVEMENT OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN THE INVASION OF CULTURED-MAMMALIAN-CELLS BY SALMONELLA-TYPHIMURIUM
INVOLVEMENT OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR IN THE INVASION OF CULTURED-MAMMALIAN-CELLS BY SALMONELLA-TYPHIMURIUM
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DOI：
10.1038/357588a0
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发表时间：
1992-06-18
期刊：
影响因子：
64.8
通讯作者：
HAYMAN, MJ
中科院分区：
文献类型：
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作者：
GALAN, JE;PACE, J;HAYMAN, MJ
沙门氏菌感染仍然是一个主要的世界性健康问题1。所有沙门氏菌共同的一个基本致病特征是它们能够穿透肠上皮细胞，这些细胞通常是非吞噬细胞2。沙门氏菌内化到哺乳动物细胞中被认为是受体介导的现象，并且培养的上皮细胞的侵入取决于几种沙门氏菌基因，但对参与这种相互作用的宿主决定簇一无所知3-6。蛋白质酪氨酸磷酸化跟随许多细胞表面受体的刺激，以启动刺激细胞反应的信号转导途径7。我们在这里报告，入侵培养的Henle-407细胞由鼠伤寒沙门氏菌诱导的表皮生长因子（EGF）受体的酪氨酸磷酸化。与此相反，S.由于invA基因突变而在侵袭方面有缺陷的鼠伤寒沙门氏菌不能诱导这种磷酸化。在培养的Henle-407细胞中加入EGF可使侵袭缺陷型S.尽管它不引起粘附的、但非侵入性的大肠杆菌菌株的内化。这一结果表明，EGF受体的刺激参与了S.鼠伤寒。
SALMONELLA infection continues to be a major world-wide health problem 1. One essential pathogenic feature common to all Salmonella is their ability to penetrate the cells of the intestinal epithelium which are normally non-phagocytic 2. The internalization of Salmonella into mammalian cells is thought to be a receptor-mediated phenomenon and the invasion of cultured epithelial cells depends on several Salmonella genes, but nothing is known about the host determinants participating in this interaction 3-6. Protein tyrosine phosphorylation follows stimulation of many cell-surface receptors to initiate signal transduction pathways that stimulate cellular responses 7. We report here that invasion of cultured Henle-407 cells by Salmonella typhimurium induces the tyrosine phosphorylation of the epidermal growth factor (EGF) receptor. In contrast, an isogenic strain of S. typhimurium that is defective in invasion owing to a mutation in the invA gene is unable to induce such phosphorylation. Addition of EGF to cultured Henle-407 cells allowed the internalization of the invasion-defective S. typhimurium invA mutant although it did not cause the internalization of an adherent, but non-invasive, strain of Escherichia coli. This result indicates that stimulation of the EGF receptor is involved in the invasion of cultured Henle-407 cells by S. typhimurium.