Priming nanoparticle-guided diagnostics and therapeutics towards human organs-on-chips microphysiological system.
Priming nanoparticle-guided diagnostics and therapeutics towards human organs-on-chips microphysiological system.
复制标题
启动纳米颗粒引导的诊断和对人体器官微生物生理系统的疗法。
DOI：
10.1186/s40580-016-0084-8
复制
发表时间：
2016
期刊：
影响因子：
11.7
通讯作者：
Kim HJ
中科院分区：
文献类型：
--
作者：
Choi JH;Lee J;Shin W;Choi JW;Kim HJ
纳米技术和生物工程在过去的几十年里融合在一起，多功能纳米颗粒（NPs）在临床和生物医学领域的应用已经出现。已经在包括二维（2D）细胞培养物或3D类器官模型的常规体外培养模型中验证了引发以检测疾病特异性生物标志物或递送生物药物化合物的NP。然而，缺乏具有强烈人体生理相关性的实验模型阻碍了对NP的安全性和功能性的准确验证。可替代地，仿生人类“芯片上器官”微生理系统已经概括了人类器官微环境的机械动态3D组织界面，其中可以更准确地验证NP及其缀合物的运输、细胞毒性、生物相容性和治疗功效。最后，NP引导的诊断检测和靶向纳米治疗剂与人类器官芯片的结合可以提供一种新的途径来加速基于NP的药物开发过程以及快速检测与病理生理过程相关的细胞分泌体。
Nanotechnology and bioengineering have converged over the past decades, by which the application of multi-functional nanoparticles (NPs) has been emerged in clinical and biomedical fields. The NPs primed to detect disease-specific biomarkers or to deliver biopharmaceutical compounds have beena validated in conventional in vitro culture models including two dimensional (2D) cell cultures or 3D organoid models. However, a lack of experimental models that have strong human physiological relevance has hampered accurate validation of the safety and functionality of NPs. Alternatively, biomimetic human “Organs-on-Chips” microphysiological systems have recapitulated the mechanically dynamic 3D tissue interface of human organ microenvironment, in which the transport, cytotoxicity, biocompatibility, and therapeutic efficacy of NPs and their conjugates may be more accurately validated. Finally, integration of NP-guided diagnostic detection and targeted nanotherapeutics in conjunction with human organs-on-chips can provide a novel avenue to accelerate the NP-based drug development process as well as the rapid detection of cellular secretomes associated with pathophysiological processes.