Comparative phytochemical analysis and antibacterial efficacy of in vitro and in vivo extracts from East Indian sandalwood tree (Santalum album L.)
Comparative phytochemical analysis and antibacterial efficacy of in vitro and in vivo extracts from East Indian sandalwood tree (Santalum album L.)
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DOI：
10.1111/lam.12005
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发表时间：
2012-12-01
影响因子：
2.4
通讯作者：
Dey, S.
中科院分区：
文献类型：
--
作者：
Misra, B. B.;Dey, S.
檀香油已被发现在许多传统药物治疗应用，如中国传统药物和阿育吠陀。然而，在文献中没有比较帐户，集中在体外和体内树样本衍生提取物。从离体样品（愈伤组织、体胚和幼苗）和体内非产油幼树和产油成熟树的叶片中获得二氯甲烷和甲醇联合提取物。提取物的植物化学评价表明，该树富含萜类化合物、皂苷、酚类物质和单宁。采用圆盘扩散法、琼脂斑点法和薄层生物自显影法对9株革兰氏阴性菌和5株革兰氏阳性菌进行筛选，比较5种提取物与檀香油的抑菌性能。筛选出的大多数试验微生物对檀香油的最小抑制浓度（MIC）在0.078 ~ 5 μ g ml（-1）范围内。生物自传结果表明，体细胞胚提取物和檀香油中存在潜在的抗菌成分。在筛选的提取物中，体胚提取物的抑菌活性最强，仅次于檀香油和成熟树叶提取物。本文的研究结果还表明，除了檀香油，檀香树在发育阶段的其他部分也富含抗菌原理。
Sandalwood oil has been found in numerous therapeutic applications in traditional medicines such as Chinese traditional medicine and Ayurveda. However, there are no comparative accounts available in the literature that focused on in vitro and in vivo tree sample-derived extracts. Combined dichloromethane and methanol extracts were obtained from in vitro samples, that is, callus, somatic embryo and seedlings, and in vivo from leaves of non-oil-yielding young and oil-yielding matured trees. Phytochemical evaluation of the extracts reveals that the tree is rich in terpenoids, saponin, phenolics and tannins. The antibacterial properties of the five extracts were compared with sandalwood oil by screening against nine Gram-negative and five Gram-positive bacterial strains by disc diffusion, agar spot and TLC bioautography methods. Minimum inhibitory concentration (MIC) for sandalwood oil was determined to be in the range of 0.078-5 mu g ml(-1) for most of the test micro-organisms screened. Bioautography results indicated the presence of potential antimicrobial constituents in somatic embryo extracts and sandalwood oil. Among the extracts screened, the somatic embryo extracts showed the strongest antibacterial activity comparable only with sandalwood oil and matured tree leaves' extract. The findings presented here also suggest that apart from sandalwood oil, other parts of this tree across developmental stages are also enriched with antibacterial principles.