Aldosterone induces angiotensin-converting-enzyme gene expression in cultured neonatal rat cardiocytes
Aldosterone induces angiotensin-converting-enzyme gene expression in cultured neonatal rat cardiocytes
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DOI：
10.1161/01.cir.104.2.137
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发表时间：
2001-07-10
期刊：
影响因子：
37.8
通讯作者：
Ogawa, H
中科院分区：
文献类型：
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作者：
Harada, E;Yoshimura, M;Ogawa, H
背景：心脏肾素-血管紧张素-醛固酮系统在衰竭的心脏中被激活与疾病的严重程度成正比。我们推测该系统中可能存在正反馈机制，并有助于心力衰竭的进展。方法和结果：为了验证这一假设，我们检测了血管紧张素Il或醛固酮是否诱导培养的新生大鼠心室心肌细胞血管紧张素转换酶（ACE） mRNA的表达。实时逆转录-聚合酶链反应检测并定量ACE mRNA的表达。暴露于血管紧张素II (10(-5) mol/L) 24小时对ACE mRNA表达无显著影响（与对照组相比为0.7 +/-0.5倍，P=NS），但与之相似的醛固酮（10(-5)mol/L）处理诱导了23.3 +/- 7.9倍的增加（P =NS）
Background-The cardiac renin-angiotensin-aldosterone system is activated in failing hearts in proportion to the severity of the disease. We hypothesized that a positive feedback mechanism might exist within this system and contribute to the progression of the heart failure.Methods and Results-To test this hypothesis, we examined whether angiotensin Il or aldosterone induces the expression of angiotensin-converting-enzyme (ACE) mRNA in cultured neonatal rat ventricular cardiocytes. Expression of ACE mRNA was detected and quantified using real-time reverse transcription-polymerase chain reaction. Exposure to angiotensin II (10(-5) mol/L) for 24 hours had no significant effect on the expression of ACE mRNA (0.7 +/-0.5-fold versus control, P=NS), but similar treatment with aldosterone (10(-5) mol/L) induced a 23.3 +/- 7.9-fold increase (P