The nitric oxide/cyclic GMP pathway in the olfactory processing system of the terrestrial slug Limax marginatus
The nitric oxide/cyclic GMP pathway in the olfactory processing system of the terrestrial slug Limax marginatus
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DOI：
10.2108/zsj.19.15
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发表时间：
2002-01-01
影响因子：
0.9
通讯作者：
Ito, E
中科院分区：
文献类型：
--
作者：
Fujie, S;Aonuma, H;Ito, E
为了检查陆生蛞蝓的触手和前脑叶（嗅觉处理中心）中一氧化氮（NO）生成细胞和NO反应细胞的分布，我们应用了NADPH黄酶（NADPH-d）组织化学和NO诱导的环鸟苷酸（cGMP）样免疫组织化学。我们发现 NADPH-d 反应性细胞/纤维和 cGMP 样免疫反应性细胞/纤维不同，但它们在触手和前脑叶中彼此相邻。然后，当嗅觉神经被电刺激以替代气味刺激时，我们使用 NO 敏感电极测量了前脑叶周围产生的 NO 浓度。刺激嗅觉神经引起纳摩尔水平的NO浓度增加，这表明纳摩尔浓度的NO与人工血红素基团的结合激活可溶性鸟苷酸环化酶。因此，结合之前报道的生理数据，我们的结果表明 NO/cGMP 途径参与蛞蝓的嗅觉处理。
To examine the distribution of nitric oxide (NO)-generative cells and NO-responsive cells in the tentacles and procerebral lobes (olfactory processing center) of terrestrial slugs, we applied NADPH diaphorase (NADPH-d) histochemistry and NO-induced cyclic GMP (cGMP)-like immunohistochemistry. We found that NADPH-d reactive cells/fibers and cGMP-like immunoreactive cells/fibers were different, but they were localized adjacent to each other, in both the tentacles and the procerebral lobes. Then, we measured the concentration of NO that was generated around the procerebral lobes using an NO sensitive electrode, when the olfactory nerve was electrically stimulated as a replacement for an odorant stimulus. Stimulation of the olfactory nerve evoked an increase in NO concentration at nanomolar levels, suggesting that binding of nanomolar concentrations of NO to the prosthetic heme group activates soluble guanylyl cyclase. Taken together with previously reported physiological data, our results, therefore, showed that the NO/cGMP pathways are involved in slug olfactory processing.