Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria
Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria
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DOI：
10.1111/j.1365-2672.2005.02789.x
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发表时间：
2006-02-01
影响因子：
4
通讯作者：
Du, Z
中科院分区：
文献类型：
--
作者：
Si, W;Gong, J;Du, Z
目的：评价精油和结构相关的合成食品添加剂在减少猪肠道细菌致病菌方面的潜力。方法与结果：采用抑菌法测定精油/化合物的抑菌活性。66种精油/化合物对沙门氏菌DT104和大肠杆菌O157:H7具有b> = 80%的抑制作用，其中9种精油/化合物被进一步研究。大部分精油/化合物对鼠伤寒沙门氏菌DT104、大肠杆菌O157:H7和大肠杆菌K88菌毛均有较高的抑菌效果，对乳酸菌和双歧杆菌抑菌效果较差。当与猪盲肠食糜混合时，这些油/化合物保留了对大肠杆菌O157:H7的功效。此外，它们还能显著抑制食糜中的大肠杆菌和大肠菌群，但对乳酸菌和厌氧菌总数的影响不大。结论：一些精油/化合物在减少猪肠道的人和动物细菌病原体方面表现出良好的潜力，包括疗效、对低pH值的耐受性和对细菌病原体的选择性。研究的意义和影响：本研究已经确定了用于体内研究的候选精油/化合物，以开发抗生素替代品，以减少猪肠道中人类和动物细菌病原体。
Aims: To assess the potential of essential oils and structurally related synthetic food additives in reducing bacterial pathogens in swine intestinal tract.Methods and Results: The antimicrobial activity of essential oils/compounds was measured by determining the inhibition of bacterial growth. Among 66 essential oils/compounds that exhibited >= 80% inhibition towards Salmonellatyphimurium DT104 and Escherichia coli O157:H7, nine were further studied. Most of the oils/compounds demonstrated high efficacy against S. typhimurium DT104, E. coli O157:H7, and E. coli with K88 pili with little inhibition towards lactobacilli and bifidobacteria. They were also tolerant to the low pH. When mixed with pig cecal digesta, these oils/compounds retained their efficacy against E. coli O157:H7. In addition, they significantly inhibited E. coli and coliform bacteria in the digesta, but had little effect on the total number of lactobacilli and anaerobic bacteria.Conclusions: Some essential oils/compounds demonstrated good potential, including efficacy, tolerance to low pH, and selectivity towards bacterial pathogens, in reducing human and animal bacterial pathogens in swine intestinal tract.Significance and Impact of the Study: This study has identified candidates of essential oils/compounds for in vivo studies to develop antibiotic substitutes for the reduction of human and animal bacterial pathogens in swine intestinal tract.