HIGH-FREQUENCY TRANSFORMATION OF PENICILLIUM-CHRYSOGENUM
HIGH-FREQUENCY TRANSFORMATION OF PENICILLIUM-CHRYSOGENUM
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DOI：
10.1038/nbt0587-494
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发表时间：
1987-05-01
期刊：
影响因子：
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通讯作者：
MARTIN, JF
中科院分区：
文献类型：
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作者：
CANTORAL, JM;DIEZ, B;MARTIN, JF
我们报道了利用携带粗链霉菌pyr4基因的质粒载体对产黄青霉素青霉菌进行高频转化的研究。转化为稳定的尿嘧啶非依赖性与获得高分子形式的转化DNA有关，这些转化DNA不是载体的简单多聚体。其中一株转化菌株的5‘-单磷酸脱羧酶活性(由pyr4基因编码)比野生型菌株高5倍，表明转化载体中的基因在青霉菌中得到了高效表达。
We report high–frequency transformation of penicillin–producing strains ofPenicillium chrysogenumusing plasmid vectors carrying thepyr4 gene ofNeurospora crassa. Transformation to stable uracil independence is associated with acquisition of transforming DNA in high molecular forms that are not simple multimers of the vector. A transformant strain showed a fivefold higher orotidine–5′–monophosphate decarboxylase activity (coded by thepyr4 gene) than the wild type strain, indicating that the genes carried in the transforming vector are efficiently expressed inPenicillium.