Structural characteristics and barrier properties of the choroid plexuses in developing brain of the opossum (Monodelphis domestica)
Structural characteristics and barrier properties of the choroid plexuses in developing brain of the opossum (Monodelphis domestica)
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DOI：
10.1002/cne.10661
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发表时间：
2003-06-09
影响因子：
2.5
通讯作者：
Saunders, NR
中科院分区：
文献类型：
--
作者：
Ek, CJ;Habgood, MD;Saunders, NR
研究了家鼠血脑脊液屏障处脉络膜丛的结构和功能发育。与传统的真动物物种相比，有袋动物在出生时非常不成熟。收集每个脑室早期发育阶段的脉络膜丛组织进行光镜和电镜观察。在发育过程中，脉络膜上皮由假层状变为立方层。单个上皮细胞似乎经历了类似的成熟过程，尽管每个神经丛之间和内部的时间不同。脉络膜上皮细胞在发育过程中的超微结构变化包括线粒体和微绒毛数量的增加以及内质网结构的改变。随着年龄的增长，神经丛的核心也有变化。相反，上皮细胞之间紧密连接的结构似乎不随成熟而改变。此外，脂不溶性分子从血液穿过脉络膜丛渗透到脑脊液的途径使用小生物素-葡聚糖进行了检测。这表明紧密连接在早期发育中已经通过阻止示踪剂的细胞外运动形成功能屏障。细胞内染色显示这些分子可能通过上皮细胞从血液到脑脊液的跨细胞途径。除了缺乏富糖阶段外，负鼠神经丛发育中的细胞变化似乎与其他物种相似，这表明负鼠神经丛发育是研究哺乳动物脉络膜神经丛发育的良好模型。(C) 2003 Wiley-Liss, Inc。
The structural and functional development of the choroid plexuses, the site of the blood-cerebrospinal fluid (CSF) barrier, in an opossum (Monodelphis domestica) was studied. Marsupial species are extremely immature at birth compared with more conventional eutherian species. Choroid plexus tissue of each brain ventricle, from early stages of development, was collected for light and electron microscopy. During development, the choroidal epithelium changes from a pseudostratified to a cuboidal layer. Individual epithelial cells appear to go through a similar maturation process even though the timing is different between and within each plexus. The ultrastructural changes during development in the choroidal epithelial cells consist of an increase in the number of mitochondria and microvilli, and changes in structure of endoplasmic reticulum. There are also changes in the core of plexuses with age. In contrast, the structure of the tight junctions between epithelial cells does not appear to change with maturation. In addition, the route of penetration for lipid insoluble molecules from blood to CSF across the choroid plexuses was examined using a small biotin-dextran. This showed that the tight junctions already form a functional barrier in early development by preventing the paracellular movement of the tracer. Intracellular staining shows that there may be a transcellular route for these molecules through the epithelial cells from blood to CSF. Apart from lacking a glycogen-rich stage, cellular changes in the developing opossum plexus seem to be similar to those in other species, demonstrating that this is a good model for studies of mammalian choroid plexus development. (C) 2003 Wiley-Liss, Inc.