The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila.
The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila.
复制标题
pH 诱导的果蝇动力蛋白轻链 LC8 单体的溶液结构。
DOI：
10.1110/ps.03462204
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Barbar,Elisar
中科院分区：
文献类型：
--
作者：
Makokha,Moses;Huang,YuanpengJanet;Montelione,Gaetano;Edison,ArthurS;Barbar,Elisar
The structure ofDrosophilaLC8 pH‐induced monomer has been determined by NMR spectroscopy using the program AutoStructure. The structure at pH 3 and 30°C is similar to the individual subunits of mammalian LC8 dimer with the exception that a β strand, which crosses between monomers to form an intersubunit β‐sheet in the dimer, is a flexible loop with turnlike conformations in the monomer. Increased flexibility in the interface region relative to the rest of the protein is confirmed by dynamic measurements based on15N relaxation. Comparison of the monomer and dimer structures indicates that LC8 is not a domain swapped dimer.
