Genetic Dissection of Adult Plant Resistance to Sharp Eyespot Using an Updated Genetic Map of Niavt14 x Xuzhou25 Winter Wheat Recombinant Inbred Line Population
Genetic Dissection of Adult Plant Resistance to Sharp Eyespot Using an Updated Genetic Map of Niavt14 x Xuzhou25 Winter Wheat Recombinant Inbred Line Population
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利用 Niavt14 x Xuzhou25 冬小麦重组自交系群体的更新遗传图谱对成年植株对尖眼斑的抗性进行遗传解析
DOI：
10.1094/pdis-09-20-1924-re
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发表时间：
2021-04-01
期刊：
影响因子：
4.5
通讯作者：
Wu, Jizhong
中科院分区：
文献类型：
--
作者：
Liu, Caiyun;Guo, Wei;Wu, Jizhong
小麦纹枯病是一种主要由禾谷丝核菌引起的土传病害，严重威胁着世界小麦生产。小麦对纹枯病的遗传抗性是减少杀菌剂使用和农业生产投入的潜在途径。为了明确Niavt 14抗纹枯病的遗传基础，构建了由Niavt 14 ×徐州25的215个F-8株系组成的重组自交系群体。早期的连锁图（148个简单序列重复标记）更新为5，792个多态性的AffyphyxAxiom 55 K单核苷酸多态性的5，684.2厘摩与1，406个非冗余标记的新地图。新的连锁图谱覆盖了普通小麦的全部21条染色体，并与IWGSC RefSeq v1.0基因组显示出良好的共线性。利用连续5个生长季的田间试验和1个温室试验的成株反应数据，进行了抗纹枯病QTL定位。在2B和7 D染色体上定位了2个稳定的QTL，在1D、6D和7A染色体上定位了3个新的稳定QTL，它们与物候和株高QTL无关，可解释4.0 ~ 17.5%的表型变异。1D、2B、6D和7A QTL在南方冬麦区384份地方品种和269份优良品种中的频率较低，分别为0 ~ 10%和5 ~ 23%。这些QTL可用于小麦抗纹枯病的分子标记辅助育种。
Wheat sharp eyespot, a disease mainly caused by soilborne fungus Rhizoctonia cerealis, is a threat to world wheat production. Wheat's genetic resistance to sharp eyespot is a potential approach to reducing the application of fungicides and farming practice inputs. To identify the genetic basis of sharp eyespot resistance in Niavt14, a recombinant inbred line population comprising 215 F-8 lines from Niavt14 x Xuzhou25, was developed. An earlier linkage map (148 simple sequence repeat markers) was updated with 5,792 polymorphic Affymetrix Axiom 55K single-nucleotide polymorphisms to a new map of 5,684.2 centimorgans with 1,406 nonredundant markers. The new linkage map covered all 21 chromosomes of common wheat and showed a good collinearity with the IWGSC RefSeq v1.0 genome. We conducted quantitative trait locus (QTL) mapping for sharp eyespot resistance using the adult plant response data from the field of five consecutive growing seasons and one greenhouse test. Two stable QTL on chromosomes 2B and 7D that were identified in the previous study were confirmed, and three novel, stable QTL, explaining 4.0 to 17.5% phenotypic variation, were mapped on 1D, 6D, and 7A, which were independent of QTL for phenology and plant height. The QTL on 1D, 2B, 6D, and 7A showed low frequencies in 384 landraces (0 to 10%) and 269 elite cultivars (5 to 23%) from the southern winter wheat region and the Yellow and Huai River Valley facultative wheat region in China, respectively. These identified QTL could be used in wheat breeding programs for improving sharp eyespot resistance through marker-assisted selection.