High titer glucagon antisera.
High titer glucagon antisera.
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高滴度胰高血糖素抗血清。
DOI：
10.1210/endo-100-2-367
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发表时间：
1977
期刊：
影响因子：
4.8
通讯作者：
J. Markese
中科院分区：
文献类型：
--
作者：
H. Tager;Matthew Hohenboken;J. Markese
用二氟二硝基苯与白蛋白偶联的胰高血糖素免疫兔，抗体效价迅速上升。在免疫测定的条件下，免疫100天后，四只动物中两只的血清和另外两只动物的大约4nL的血清需要结合10pg的[125I]碘高血糖素的50%。这两种效价较高的抗血清的解离常数约为1×10~(-10)M，与激素的结合容量约为50微克/毫升。竞争结合试验表明，这两种抗血清与肠内提取的类胰高血糖素免疫活性多肽的交叉反应率均不超过2.5%。相比之下，母鸡产生的抗血清与肠道物质有反应，每毫升仅结合0.3杯胰高血糖素。然而，特异性和非特异性抗血清与选定的胰腺高血糖素片段的反应能力没有一致的差异。
Antibody titers in rabbits immunized with glucagon conjugated to albumin using difluorodinitrobenzene rose rapidly. Under conditions of immunoassay, less than 2 nl of serum from two of four animals and approximately 4 nl from the other two was required to bind 50% of the 10 pg of [125I]iodoglucagon 100 days after immunization. The dissociation constants of the two higher titer antisera for glucagon were approximately 1 x 10(-10)M, and their binding capacities for the hormone, about 50 mug/ml. Competitive binding assays showed that neither of these antisera cross-reacts with the glucagon-like, immunoreactive peptides extracted from intestine to greater than 2.5%. In contrast, hens produced antisera which were reactive with the intestinal material and which bound only 0.3 mug of glucagon per ml. There were no consistent differences, however, in the abilities of specific and non-specific antisera to react with selected fragments of pancreatic glucagon.