Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering.
Electroactive Tissue Scaffolds with Aligned Pores as Instructive Platforms for Biomimetic Tissue Engineering.
复制标题
DOI：
10.3390/bioengineering2010015
复制
发表时间：
2015-01-14
期刊：
影响因子：
--
通讯作者：
Schmidt CE
中科院分区：
文献类型：
--
作者：
Hardy JG;Cornelison RC;Sukhavasi RC;Saballos RJ;Vu P;Kaplan DL;Schmidt CE
体内组织是分层结构的复合材料，具有组织特定的化学和拓扑特性。在这里，我们报告了通过从可生物降解的聚合物支架（聚己内酯，PCL）中溶解牺牲性超分子聚合物晶体模板（尿素）而产生的具有宏观孔的组织支架的制备。此外，我们报告了一种在 PCL 内排列基于超分子聚合物的晶体的方法，并且牺牲尿素的溶解产生具有宏观孔的支架，这些孔在长的、临床相关的距离（即厘米级）上排列。这些孔充当地形线索，大鼠雪旺细胞通过与孔的长轴对齐来做出反应。在支架中生成聚吡咯（PPy）和聚（苯乙烯磺酸）（PSS）互穿网络，产生电活性组织支架，可以对支架上培养的雪旺细胞进行电刺激，从而增加神经生长因子（NGF）的产生。
Tissues in the body are hierarchically structured composite materials with tissue-specific chemical and topographical properties. Here we report the preparation of tissue scaffolds with macroscopic pores generated via the dissolution of a sacrificial supramolecular polymer-based crystal template (urea) from a biodegradable polymer-based scaffold (polycaprolactone, PCL). Furthermore, we report a method of aligning the supramolecular polymer-based crystals within the PCL, and that the dissolution of the sacrificial urea yields scaffolds with macroscopic pores that are aligned over long, clinically-relevant distances (i.e., centimeter scale). The pores act as topographical cues to which rat Schwann cells respond by aligning with the long axis of the pores. Generation of an interpenetrating network of polypyrrole (PPy) and poly(styrene sulfonate) (PSS) in the scaffolds yields electroactive tissue scaffolds that allow the electrical stimulation of Schwann cells cultured on the scaffolds which increases the production of nerve growth factor (NGF).