AIRWAY MUCOSAL BLOOD-FLOW IN HUMANS - RESPONSE TO ADRENERGIC AGONISTS
AIRWAY MUCOSAL BLOOD-FLOW IN HUMANS - RESPONSE TO ADRENERGIC AGONISTS
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DOI：
10.1164/ajrccm.149.5.8173752
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发表时间：
1994-05-01
影响因子：
24.7
通讯作者：
WANNER, A
中科院分区：
文献类型：
--
作者：
ONORATO, DJ;DEMIROZU, MC;WANNER, A
我们测量了可溶性惰性气体二甲醚（DME）从一段传导气道的吸收，以估计粘膜血流量（Q（aw））非侵入性。受试者从功能性残气量位置吸入300 ml含35% DME、8%氦气、35%氧气和余量氮气的混合气体;他们屏住呼吸5 s，然后呼出到肺量计中。在呼气过程中，记录DME和氦气的瞬时浓度以及呼出气体体积。以5、10、15和20 s的屏气时间重复该操作。我们计算Q（aw）使用DME浓度的时间依赖性降低与80和130 mi（代表声门远端的解剖死腔段）之间的呼气体积分数中的氦浓度和平均DME浓度。在10名健康的非吸烟者中，平均（+/- SE）Q（aw）为8.0 +/- 1.3 ml/min，或8 +/- 2 μ l/min/cm（2）粘膜表面。我们在绵羊的验证实验中获得了12 +/- 3 μ l/min/cm（2）的值。在7名受试者中，吸入甲氧胺（雾化剂量10 mg）导致平均Q（aw）下降65 +/- 19%（p < 0.05），沙丁胺醇（雾化剂量2.5 mg）增加92 +/- 17%（p < 0.05），最大变化发生在吸入后即刻或15分钟。我们的结论是，DME摄取方法是一个可以接受的非侵入性手段估计气道粘膜血流在人类和血管活性物质的修改。
We measured the uptake of the soluble inert gas dimethyl ether (DME) from a segment of the conducting airways to estimate mucosal blood flow (Q(aw)) noninvasively. The subjects inhaled, from the functional residual capacity position, a 300-ml gas mixture containing 35% DME, 8% helium, 35% oxygen, and the balance nitrogen; they held their breath for 5 s and then exhaled into a spirometer. During exhalation, the instantaneous concentrations of DME and helium were recorded together with expired gas volume. The maneuver was repeated with breathhold times of 5, 10, 15, and 20 s. We calculated Q(aw) using the time-dependent decrease in DME concentration in relation to the helium concentration in an expired volume fraction between 80 and 130 mi (representing an anatomic dead-space segment distal to the glottis) and the mean DME concentration. In 10 healthy nonsmokers, mean (+/- SE) Q(aw) was 8.0 +/- 1.3 ml/min, or 8 +/- 2 mu l/min/cm(2) mucosal surface. We obtained a value of 12 +/- 3 mu l/min/cm(2) in a validation experiment in sheep. Inhaled methoxamine (nebulized dose 10 mg) caused a 65 +/- 19% decrease (p < 0.05), and albuterol (nebulized dose 2.5 mg) a 92 +/- 17% increase (p < 0.05), in mean Q(aw) in seven subjects, with the maximum changes occurring immediately or 15 min postinhalation. We conclude that the DME uptake method is an acceptable noninvasive means of estimating airway mucosal blood flow in humans and its modification by vasoactive substances.