A global assembly line for cyanobactins
A global assembly line for cyanobactins
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DOI：
10.1038/nchembio.84
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发表时间：
2008-06-01
影响因子：
14.8
通讯作者：
Schmidt, Eric W.
中科院分区：
文献类型：
--
作者：
Donia, Mohamed S.;Ravel, Jacques;Schmidt, Eric W.
从海洋海鞘、海绵和不同属的蓝藻中发现了100多个含有杂环残基的环肽。在这里，我们报告了一条流水线，负责在共生和自由生活的蓝藻中生物合成这些不同的多肽，现在被称为氰基结合蛋白。通过比较五个新的氰基丁酸菌素生物合成簇，我们利用基因工程技术在大肠杆菌培养中产生了抗癌前候选蛋白Trunkamide。
More than 100 cyclic peptides harboring heterocyclized residues are known from marine ascidians, sponges and different genera of cyanobacteria. Here, we report an assembly line responsible for the biosynthesis of these diverse peptides, now called cyanobactins, both in symbiotic and free-living cyanobacteria. By comparing five new cyanobactin biosynthetic clusters, we produced the prenylated antitumor preclinical candidate trunkamide in Escherichia coli culture using genetic engineering.