ANALYSIS OF THE CD1 CLUSTER IN SHEEP
ANALYSIS OF THE CD1 CLUSTER IN SHEEP
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DOI：
10.1016/0165-2427(91)90099-x
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发表时间：
1991-01-01
影响因子：
1.8
通讯作者：
HOPKINS, J
中科院分区：
文献类型：
--
作者：
DUTIA, BM;HOPKINS, J
提交给第一届国际牛、绵羊和山羊白细胞分化抗原研讨会的抗CD1单克隆抗体，测试了其在绵羊皮肤、胸腺和淋巴结上的反应性，以及与绵羊传出和传入淋巴液和外周血的反应性。 除了 20-27 之外，它们都对相同的细胞群进行染色。 抗体沉淀的分子具有46 000表观分子量的重链和14 000表观分子量的轻链。 VPM5和CC14抗原通过亲和层析纯化。 所有抗体都与这些分子发生交叉反应。 结果显示20-27与其他抗体识别相同的分子，表明20-27是泛CD1单克隆抗体，其他单克隆抗体是人CD1b分子的同源物。
The anti-CD1 monoclonal antibodies submitted to the 1st International Workshop on Leucocyte Differentiation Antigens of Cattle, Sheep and Goats were tested for their reactivity on sheep skin, thymus and lymph node and for their reactivity with sheep efferent and afferent lymph and peripheral blood. With the exception of 20-27 they all stained that same cell populations. The antibodies precipitated molecules with a heavy chain of 46 000 apparent molecular weight and a light chain of 14 000 apparent molecular weight. VPM5 and CC14 antigens were purified by affinity chromatography. All the antibodies cross-reacted with these molecules. The results show that 20-27 recognises the same molecules as the other antibodies and suggest that 20-27 is a pan CD1 monoclonal antibody and the other monoclonal antibodies are homologues of the human CD1b molecules.