Molecular cloning and functional characterization of OsJAG gene based on a complete-deletion mutant in rice (Oryza sativa L.)
Molecular cloning and functional characterization of OsJAG gene based on a complete-deletion mutant in rice (Oryza sativa L.)
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DOI：
10.1007/s11103-010-9703-7
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发表时间：
2010-12-01
影响因子：
5.1
通讯作者：
Wu, Weiren
中科院分区：
文献类型：
--
作者：
Duan, Yuanlin;Diao, Zhijuan;Wu, Weiren
本文报道了水稻OsJAG基因的定位克隆及其功能研究。我们的工作的优点是，我们使用了一个真正的无效突变体，其中野生型等位基因被完全删除。这使我们能够准确地鉴定突变表型，而不受靶基因的残余功能的干扰。OsJAG基因是一个多效性基因，在植物中广泛表达，在营养生长期和生殖生长期均发挥作用。但它的主要和关键作用是在调节所有花器官的发育，特别是在指定雄蕊的身份。有趣的是，OsJAG不影响花器官原基的数量，也不影响每轮花器官的数量，表明OsJAG不影响花器官原基的发生，但影响所有花器官原基发生后的发育命运。OsJAG功能的缺失导致花器官发育不良，如外稃和内稃退化，浆片伸长，雌蕊畸形和不育。雄蕊似乎对突变更敏感。突变体小花中的6个雄蕊全部转化为6个雌蕊状器官，发育于第3轮雄蕊的假定位置。
In this article, we report an independent work of positional cloning and functional characterization of OsJAG gene in rice. The merit of our work is that we used a genuine null mutant, in which the wild-type allele was completely deleted. This allowed us to identify the mutant phenotypes accurately without the interference of residual function of the target gene. OsJAG is an important gene with pleiotropy, expressing almost throughout the plant and acting in both vegetative phase and reproductive phase. But its main and crucial roles are in regulating the development of all floral organs, especially in specifying the identity of stamens. Interestingly, OsJAG does not affect the number of floral organ primordial and so of floral organs in each whorl, suggesting that OsJAG does not influence the initiation of floral organ primordia, but affect the developmental fate of all floral organs after their primordia have initiated. Loss of OsJAG function results in maldevelopment of all floral organs, such as degenerated lemma and palea, elongated lodicules and deformed and sterile pistil. The stamen appears to be more sensitive to the mutation. All the six stamens in a mutant floret were thoroughly transformed into six pistil-like organs developed at the presumptive positions of the stamens in whorl 3.