Priming of the Respiratory Tract with Immunobiotic Lactobacillus plantarum Limits Infection of Alveolar Macrophages with Recombinant Pneumonia Virus of Mice (rK2-PVM)
Priming of the Respiratory Tract with Immunobiotic Lactobacillus plantarum Limits Infection of Alveolar Macrophages with Recombinant Pneumonia Virus of Mice (rK2-PVM)
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DOI：
10.1128/jvi.02279-15
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发表时间：
2016-01-01
影响因子：
5.4
通讯作者：
Rosenberg, Helene F.
中科院分区：
文献类型：
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作者：
Dyer, Kimberly D.;Drummond, Rebecca A.;Rosenberg, Helene F.
小鼠肺炎病毒（PVM）是一种天然的啮齿类病原体，其在支气管上皮细胞中复制并再现与人呼吸道合胞病毒相关的更严重形式的疾病的许多临床和病理特征。为了在体内急性感染期间追踪病毒-靶细胞相互作用，我们开发了rK 2-PVM，基于细菌人工染色体的重组PVM菌株J3666，其并入荧光标签单体Katushka 2（mKATE 2）。rK 2-PVM病原体促进BALB/c小鼠的致死性感染，并促进特征性细胞因子产生和白细胞向肺实质的募集。使用重组病毒，我们首次证明PVM感染的树突状细胞（DC; CD 11 c（+）主要组织相容性复合物II类+）和肺泡巨噬细胞（AM; CD 11 c（+）唾液酸结合免疫球蛋白样凝集素F+）在体内，同样检测mKATE 2（+）DC在纵隔淋巴结感染的小鼠。AM支持活跃的病毒复制和感染性病毒体的产生。此外，我们报道了用免疫生物素植物乳杆菌（一种导致对急性呼吸道病毒感染的致死性炎症后遗症的保护的方案）引发呼吸道，导致中性粒细胞、DC和淋巴细胞响应rK 2-PVM而向肺的不同募集，并且从类似于40%减少到20%。
Pneumonia virus of mice (PVM) is a natural rodent pathogen that replicates in bronchial epithelial cells and reproduces many clinical and pathological features of the more severe forms of disease associated with human respiratory syncytial virus. In order to track virus-target cell interactions during acute infection in vivo, we developed rK2-PVM, bacterial artificial chromosome-based recombinant PVM strain J3666 that incorporates the fluorescent tag monomeric Katushka 2 (mKATE2). The rK2-PVM pathogen promotes lethal infection in BALB/c mice and elicits characteristic cytokine production and leukocyte recruitment to the lung parenchyma. Using recombinant virus, we demonstrate for the first time PVM infection of both dendritic cells (DCs; CD11c(+) major histocompatibility complex class II+) and alveolar macrophages (AMs; CD11c(+) sialic acid-binding immunoglobulin-like lectin F+) in vivo and likewise detect mKATE2(+) DCs in mediastinal lymph nodes from infected mice. AMs support both active virus replication and production of infectious virions. Furthermore, we report that priming of the respiratory tract with immunobiotic Lactobacillus plantarum, a regimen that results in protection against the lethal inflammatory sequelae of acute respiratory virus infection, resulted in differential recruitment of neutrophils, DCs, and lymphocytes to the lungs in response to rK2-PVM and a reduction from similar to 40% to