Structural Characterization of Chlorophyll-a by High Resolution Tandem Mass Spectrometry
Structural Characterization of Chlorophyll-a by High Resolution Tandem Mass Spectrometry
复制标题
通过高分辨率串联质谱法表征叶绿素-a 的结构
DOI：
10.1007/s13361-013-0577-1
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发表时间：
2013
影响因子：
3.2
通讯作者：
P. O’Connor
中科院分区：
文献类型：
--
作者：
Juan Wei;Huilin Li;M. Barrow;P. O’Connor
AbstractA high resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometer is used for characterizing the fragmentation of chlorophyll-a. Three tandem mass spectrometry (MS/MS) techniques, including electron-induced dissociation (EID), collisionally activated dissociation (CAD), and infrared mutiphoton dissociation (IRMPD) are applied to the singly protonated chlorophyll-a. Some previously unpublished fragments are identified unambiguously by utilizing high resolution and accurate mass value provided by the FTICR mass spectrometer. According to this research, the two long aliphatic side chains are shown to be the most labile parts, and favorable cleavage sites are proposed. Even though similar fragmentation patterns are generated by all three methods, there are much more abundant peaks in EID and IRMPD spectra. The similarities and differences are discussed in detail. Comparatively, cleavage leading to odd electron species and H• loss both seem more common in EID experiments. Extensive loss of small side groups (e.g., methyl and ethyl) next to the macrocyclic ring was observed. Coupling the high performance FTICR mass spectrometer with contemporary MS/MS techniques, especially IRMPD and EID, proved to be very promising for the structural characterization of chlorophyll, which is also suitable for the rapid and accurate structural investigation of other singly charged porphyrinic compounds.
影响因子：
7.4
作者：
Zubarev, RA;Horn, DM;McLafferty, FW
通讯作者：
McLafferty, FW
使用傅里叶变换质谱法绘制的人 p21ras 氧化翻译后修饰的详细图谱。
影响因子：
7.4
作者：
Zhao,Cheng;Sethuraman,Mahadevan;Clavreul,Nicolas;Kaur,Parminder;Cohen,RichardA;O'Connor,PeterB
通讯作者：
O'Connor,PeterB