Characterization of B cell membrane receptors of polysaccharide isolated from the root of Acanthopanax koreanum
Characterization of B cell membrane receptors of polysaccharide isolated from the root of Acanthopanax koreanum
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DOI：
10.1016/s1567-5769(03)00056-0
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发表时间：
2003-05-01
影响因子：
5.6
通讯作者：
Kim, HM
中科院分区：
文献类型：
--
作者：
Han, SB;Park, SK;Kim, HM
研究了刺五加（AK）根多糖在细胞水平上的免疫调节活性。AK直接增加B细胞增殖和抗体产生，但不影响T细胞IL-2、ifn - γ和IL-4的表达，也不影响T细胞体外增殖。由于AK分子质量大，不能穿透细胞，因此可能是AK与B细胞特异性受体表面结合引起B细胞活化。与C3H/HeN小鼠的对照细胞相比，已知具有缺陷toll样受体(TLR)-4的C3H/HeJ小鼠B细胞中的AK活性降低，这一事实证明了TLR4作为AK受体的作用。阻断TLR2、TLR4、CD19或CD79b的抗体也会降低AK的活性，但阻断CD38的抗体不会降低AK的活性，这表明在B细胞中存在AK受体谱。AK与脂多糖（LPS）的差异主要有两点。首先，LPS活性被TLR2或TLR4抗体抑制，而CD19、CD79b或CD38抗体不受抑制。另一种是LPS诱导的B细胞增殖受到LPS特异性抑制剂多粘菌素B （polymyxin B， PMB）的抑制，而AK活性不受影响。综上所述，我们的研究结果表明，AK直接激活B细胞，而不是T细胞，并且表明AK在B细胞中具有比LPS更广泛的受体谱。(C) 2003 Elsevier Science B.V.版权所有
We investigated the immunomodulatory activity of polysaccharide isolated from the root of Acanthopanax koreanum (AK) at the cellular level. AK directly increased B cell proliferation and antibody production, but did not affect the expression of IL-2, IFN-gamma or IL-4 by T cells, or T cell proliferation in vitro. Since AK cannot penetrate cells due to its large molecular mass, B cell activation may be caused by the surface binding of AK to B cell-specific receptors. The role of TLR4 as an AK receptor was shown by the fact that AK activity in B cells from C3H/HeJ mice, which are known to have a defective Toll-like receptor (TLR)-4, was found to be reduced compared with that in control cells from C3H/HeN mice. AK activity was also reduced by antibodies blocking TLR2, TLR4, CD19 or CD79b, but not by an antibody blocking CD38, which suggests AK receptor profiling in B cells. Two main differences between AK and lipopolysaccharide (LPS) were observed. First, LPS activity was inhibited by antibodies to either TLR2 or TLR4, but not by antibodies to CD19, CD79b or CD38. Another was that LPS-induced B cell proliferation was inhibited by polymyxin B (PMB), a specific inhibitor of LPS, whereas AK activity was not affected. Taken together, our results demonstrate that AK directly activates B cells, but not T cells, and suggest that AK has a broader receptor profile than LPS in B cells. (C) 2003 Elsevier Science B.V. All rights reserved.