Detection of IgA1-dominant immune complexes in peripheral blood polymorphonuclear leukocytes by double immunofluorescence in patients with IgA nephropathy.
Detection of IgA1-dominant immune complexes in peripheral blood polymorphonuclear leukocytes by double immunofluorescence in patients with IgA nephropathy.
复制标题
双免疫荧光法检测 IgA 肾病患者外周血多形核白细胞中以 IgA1 为主的免疫复合物。
作者：
Y. Tomino;M. Miura;T. Suga;M. Endoh;Y. Nomoto;H. Sakai
应用免疫荧光双标记技术检测伊加肾病患者外周血多形核细胞（PMN）中IgA 1和/或IgA 2优势免疫复合物的含量。本研究的目的是确定伊加肾病患者中性粒细胞吞噬IgA 1和/或IgA 2显性免疫复合物的患病率。对5例伊加肾病患者和10例健康成人进行了检测。结果表明，伊加肾病患者IgA 1与C3胞浆包涵体的百分比显著高于IgA 2与C3胞浆包涵体的百分比。提示伊加肾病患者外周血中性粒细胞对IgA 1优势免疫复合物有吞噬作用。
The amounts of IgA1- and/or IgA2-dominant immune complexes included in peripheral blood polymorphonuclear cells (PMN) were determined by the double immunofluorescence technique in patients with IgA nephropathy. The aim of the present study was to determine the prevalence of IgA1-and/or IgA2-dominant immune complexes phagocytized by PMN in patients with IgA nephropathy. 5 patients with IgA nephropathy and 10 healthy adults were examined. It was demonstrated that the percentages of IgA1 with C3 cytoplasmic inclusion bodies were significantly increased compared with those of IgA2 with C3 cytoplasmic inclusion bodies in patients with IgA nephropathy. It was suggested that IgA1-dominant immune complexes are phagocytized by peripheral blood PMN in patients with IgA nephropathy.