Imaging Single Nanoparticle Interactions with Human Lung Cells Using Fast Ion Conductance Microscopy
Imaging Single Nanoparticle Interactions with Human Lung Cells Using Fast Ion Conductance Microscopy
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DOI：
10.1021/nl404068p
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发表时间：
2014-03-01
期刊：
影响因子：
10.8
通讯作者：
Korchev, Yuri E.
中科院分区：
文献类型：
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作者：
Novak, Pavel;Shevchuk, Andrew;Korchev, Yuri E.
由于成像技术的限制，纳米粒子与膜过程之间在纳米尺度上的动态相互作用的实验数据仍然很少，这对纳米粒子的生物医学应用至关重要。我们能够在扫描离子电导显微镜上以15 S/帧的速度跟踪单个200 nm羧基修饰的颗粒与已鉴定的膜结构的相互作用，该显微镜经过改装，可以同时进行高速地形成像和荧光成像。这里展示的成像方法为了解纳米颗粒-细胞相互作用的复杂性打开了一扇新的窗口。
Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.