An electrochemical glutathione biosensor: ubiquinone as a transducer.
An electrochemical glutathione biosensor: ubiquinone as a transducer.
复制标题
DOI：
10.1016/j.talanta.2013.03.038
复制
发表时间：
2013-06
期刊：
影响因子：
6.1
通讯作者：
J. Ru;Jie Du;Dong-Dong Qin-Dong;Baomei Huang;Zhong-Hua Xue;Xibin Zhou;Xiaoquan Lu
中科院分区：
文献类型：
--
作者：
J. Ru;Jie Du;Dong-Dong Qin-Dong;Baomei Huang;Zhong-Hua Xue;Xibin Zhou;Xiaoquan Lu
本文首次将辅酶Q10（Ubiquinone，CoQ 10）作为传感器构建成电化学生物传感器，用于检测γ-L-谷氨酰-L-半胱氨酰-甘氨酸（glutathione，GSH）。将CoQ 10凝胶与多壁碳纳米管（MWNTs）/离子液体（IL）混合，制备CoQ 10修饰电极。在最佳条件下，利用还原型谷胱甘肽（GSH）使辅酶Q10的还原峰电流增大的特点，在pH7.00时，辅酶Q10的还原峰电流与GSH的浓度在4.0×10− 9 ~ 2.0×10− 7 molL − 1范围内呈良好的线性关系，检出限为3.2×10− 10 molL −1（S/N=3）。结果表明，该方法具有选择性好、稳定性高、灵敏度高等优点，可用于实际血样中GSH的测定。该方法为设计新型多功能GSH传感器提供了一条新的途径，GSH传感器在传感器和医学诊断领域具有独特的特点和潜在的应用前景。
In this paper, coenzyme Q10(Ubiquinone, CoQ10) was used for the first time as a transducer to construct electrochemical biosensor for effectively detecting γ-l-glutamyl-l-cysteinyl-glycine (glutathione, GSH). CoQ10modified electrode was fabricated by attaching its gel mixed with multi-walled carbon nanotubes (MWNTs)/ionic liquid (IL). In the optimum conditions, based on the increasing of reduction peak current of CoQ10caused by GSH through voltammetric technology, it was found that the peak current of CoQ10was linear with the concentration of GSH in the range from 4.0×10−9to 2.0×10−7molL−1at the pH 7.00, and the limit of detection was 3.2×10−10molL−1(S/N=3). The results revealed that this method could be used to determine GSH in actual blood samples with the superiority of excellent selectivity, high stability and sensitivity. The strategy explored here might provide a new pathway to design novel multi-function transducers for detecting GSH, which has unique characteristic and potential application in the fields of sensor and medical diagnosis.