Genetically modified fibroblasts producing NGF protect hippocampal neurons after ischemia in the rat.
Genetically modified fibroblasts producing NGF protect hippocampal neurons after ischemia in the rat.
复制标题
产生 NGF 的转基因成纤维细胞可在大鼠缺血后保护海马神经元。
DOI：
10.1097/00001756-199503000-00021
复制
发表时间：
1995
期刊：
影响因子：
1.7
通讯作者：
Breakefield,XO
中科院分区：
文献类型：
--
作者：
Pechan,PA;Yoshida,T;Panahian,N;Moskowitz,MA;Breakefield,XO
THE neuroprotective effect of nerve growth factor (NGF) on the pyramidal cells in the vulnerable CA1-CA2 sectors of the hippocampus was investigated in a rat model of transient forebrain ischemia. A genetically modified fibroblast line that secretes high levels of NGF was implanted 7 days before induction of ischemia between the hippocampal CA1-CA2 subfields in the right hemisphere. Rats were then subjected to 10 min of cerebral ischemia in a four vessel occlusion model. Morphological changes in the CA1 and CA2 subfields were evaluated 7 days after ischemia. Animals in the NGF-protected group had significantly higher numbers of normal appearing neurons in the right CA1 and CA2 regions, compared with their non-implanted left hemispheres, to non-implanted animals or to animals implanted with non-modified cells. The data confirmed that NGF can protect CA1-CA2 hippocampal neurons from ischemic damage by implantation of genetically engineered cells producing NGF.
