A CONTIGUOUS NETWORK OF DENDRITIC ANTIGEN-PRESENTING CELLS WITHIN THE RESPIRATORY EPITHELIUM
A CONTIGUOUS NETWORK OF DENDRITIC ANTIGEN-PRESENTING CELLS WITHIN THE RESPIRATORY EPITHELIUM
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DOI：
10.1159/000235107
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发表时间：
1990-02-01
期刊：
影响因子：
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通讯作者：
MCMENAMIN, PG
中科院分区：
文献类型：
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作者：
HOLT, PG;SCHONHEGRAD, MA;MCMENAMIN, PG
这项研究利用一种简单的技术在平行于基底膜的平面上切开呼吸道上皮，从而提供了上皮内细胞群的独特平面图。免疫过氧化物酶染色显示这些组织切片上皮内有Ia阳性的树突状细胞(DC)。这些DC被证明能够在体内以适合呈递给T细胞的形式结合吸入的抗原。这些细胞的战略位置和它们几乎解释了稳定状态下呼吸道上皮细胞的所有染色这一事实是令人信服的证据，表明DC网络在监测吸入性抗原方面起着“第一道防线”的作用，并进一步表明上皮内DC在呼吸道过敏和传染病中发挥着重要作用(S)。
This study utilises a simple technique to section airway epithelium in a plane parallel to the basement membrane, thus providing a unique plan view of the intra-epithelial cell populations. Immunoperoxidase staining of these tissue sections for class II major histocompatibility complex Ia antigen reveals a virtually contiguous network of Ia-positive dendritic cells (DC) within the epithelium. These DC are shown to be capable of binding inhaled antigens in vivo in a form suitable for presentation to T cells. The strategic location of these cells and the fact that they account for virtually all staining in the airway epithelium during the steady state is convincing evidence that the DC network functions as the ''first line of defence'' in surveillance for inhaled antigens and further suggests a major role for the intra-epithelial DC in allergic and infectious disease(s) in the respiratory tract.