Determination of complement of mRNA encoding inositol 1,4,5-trisphosphate receptor isoforms in rat tissues using relative-polymerase chain reaction.
Determination of complement of mRNA encoding inositol 1,4,5-trisphosphate receptor isoforms in rat tissues using relative-polymerase chain reaction.
复制标题
使用相对聚合酶链式反应测定大鼠组织中编码肌醇 1,4,5-三磷酸受体亚型的 mRNA 补体。
DOI：
10.1006/abio.2001.5174
复制
发表时间：
2001
影响因子：
2.9
通讯作者：
BeschJr,HR
中科院分区：
文献类型：
--
作者：
Gersting,JA;Bidasee,KR;DincerUD;BeschJr,HR
Materials and methods. cDNA sequences encoding rat types 1, 2, and 3 IP3R were aligned using a Lasergene Clustal analysis module (DNASTAR Inc., Madison, WI) and a region of 100% homology among the isoforms was selected for synthesis of single sense primer (Table 1). Approximately 600 bp downstream (3) another region of high homology (90%) was cho-
