Brazilein protects the brain against focal cerebral ischemia reperfusion injury correlating to inflammatory response suppression
Brazilein protects the brain against focal cerebral ischemia reperfusion injury correlating to inflammatory response suppression
复制标题
巴西素可保护大脑免受与炎症反应抑制相关的局灶性脑缺血再灌注损伤
DOI：
10.1016/j.ejphar.2006.11.059
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发表时间：
2007-03-06
影响因子：
5
通讯作者：
Du, Lijun
中科院分区：
文献类型：
--
作者：
Shen, Jia;Zhang, Hongyin;Du, Lijun
评估从苏木中分离出的巴西素对大鼠短暂性局灶性脑缺血/再灌注的神经保护作用。结果表明，脑缺血再灌注发生后给予巴西林可以缩小脑梗塞面积，改善神经功能评分。研究人员对其作用机制进行了研究，并将其归因于巴西林的抗炎作用，因为在接受巴西林治疗的缺血动物中发现促炎细胞因子（肿瘤坏死因子-α（TNF-α）和白介素-6（IL-6））的 mRNA 水平降低。为了进一步证实巴西菜素的抗炎作用，我们检测了脂多糖（LPS）诱导的小胶质细胞系BV2细胞中细胞因子的mRNA表达；巴西素治疗显着抑制了 TNF-α 和 IL-6 mRNA 表达，但未检测到白细胞介素 1 β (IL-1 β) 表达的降低。在 RAW 264.7 巨噬细胞和 BV2 细胞中测量了诱导型一氧化氮合酶 (iNOS) 产生的一氧化氮 (NO)，这是免疫细胞炎症反应的另一个指标；巴西素以剂量依赖性方式抑制两种类型细胞中 LPS 诱导的其产生。一致地，巴西莱姆也降低了 iNOS 的 mRNA 水平。总之，这些结果表明巴西素可以保护大脑免受缺血/再灌注损伤，并且抗炎作用被认为是其中的机制之一。 (c) 2006 Elsevier B.V. 保留所有权利。
Brazilein isolated from Caesalpinia sappan was evaluated for neuroprotection against transient focal cerebral ischemia/reperfusion in rats. The results showed that administration of brazilein after the onset of cerebral ischemia reperfusion can reduce the brain infarction area and improve the neurological score. The mechanisms underlying the action were investigated and attributed to the anti-inflammatory effect of brazilein, because a decrease of the mRNA level of pro-inflammatory cytokines (tumor necrosis factor-alpha(TNF-alpha) and interleukin-6 (IL-6)) was found in the ischemic animals with brazilein treatment. To further substantiate the anti-inflammatory effect of brazilein, we examined the mRNA expression of the cytokines in the lipopolysaccharide (LPS) induced microglial cell line BV2 cells; TNF-alpha and IL-6 mRNA expressions were significantly suppressed by brazilein treatment but the decrease of interleukin-1 beta (IL-1 beta) expression was not detected. Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS), another indicator of the inflammatory response of the immune cells was measured in RAW 264.7 macrophages and BV2 cells; brazilein inhibited its production induced by LPS in both types of cells in a dose-dependent manner. Consistently, the mRNA level of iNOS was also decreased by brazilem. Together, these results illustrate that brazilein can protect the brain against ischemia/reperfusion injury and the anti-inflammatory effect was believed to be one of the contributive mechanisms. (c) 2006 Elsevier B.V. All rights reserved.