Live Cell Imaging Reveals Structural Associations between the Actin and Microtubule Cytoskeleton in Arabidopsis
Live Cell Imaging Reveals Structural Associations between the Actin and Microtubule Cytoskeleton in Arabidopsis
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DOI：
10.1105/tpc.111.087940
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发表时间：
2011-06-01
期刊：
影响因子：
11.6
通讯作者：
Persson, Staffan
中科院分区：
文献类型：
--
作者：
Sampathkumar, Arun;Lindeboom, Jelmer J.;Persson, Staffan
在真核细胞中，肌动蛋白和微管 (MT) 细胞骨架网络是组织细胞内过程并促进其快速重组的动态结构。在植物细胞中，肌动蛋白丝 (AF) 和 MT 对于细胞生长和形态发生至关重要。然而，活细胞中这两种重要成分之间的动态相互作用尚未被探索。在这里，我们使用旋转盘共聚焦显微镜来剖析拟南芥中皮质 AF 和 MT 之间的相互作用和合作，利用这两个组件的荧光报告基因构建体。定量分析揭示了与皮质 MT 的位置和方向相关的 AF 动态变化。 AF 阵列的重组和重新组装取决于药物诱导解聚后的 MT，因此短 AF 最初出现与 MT 共定位，并显示出沿着 MT 的运动性。我们还观察到光诱导的 MT 重组与 AF 行为的变化同时发生。我们的结果表明，间期植物细胞中皮质肌动蛋白和 MT 细胞骨架之间存在动态相互作用。
In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes and facilitate their rapid reorganization. In plant cells, actin filaments (AFs) and MTs are essential for cell growth and morphogenesis. However, dynamic interactions between these two essential components in live cells have not been explored. Here, we use spinning-disc confocal microscopy to dissect interaction and cooperation between cortical AFs and MTs in Arabidopsis thaliana, utilizing fluorescent reporter constructs for both components. Quantitative analyses revealed altered AF dynamics associated with the positions and orientations of cortical MTs. Reorganization and reassembly of the AF array was dependent on the MTs following drug-induced depolymerization, whereby short AFs initially appeared colocalized with MTs, and displayed motility along MTs. We also observed that light-induced reorganization of MTs occurred in concert with changes in AF behavior. Our results indicate dynamic interaction between the cortical actin and MT cytoskeletons in interphase plant cells.