Amphipaths differentially modulate membrane surface deformation in rat peritoneal mast cells during exocytosis
Amphipaths differentially modulate membrane surface deformation in rat peritoneal mast cells during exocytosis
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双亲细胞在胞吐作用过程中差异调节大鼠腹膜肥大细胞的膜表面变形
DOI：
10.1159/000350079
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发表时间：
2013
影响因子：
--
通讯作者：
Kokumai T
中科院分区：
文献类型：
--
作者：
Kazama I;Maruyama Y;Takahashi S;Kokumai T
背景/目的水杨酸盐和氯丙嗪对肥大细胞释放趋化因子的影响不同。由于这些药物是两亲性的，并且优先分布在质膜的脂质双层中，它们会引起肥大细胞的一些形态变化，从而影响胞吐过程。方法采用标准的膜片钳全细胞记录技术，观察水杨酸盐和氯丙嗪对大鼠腹膜肥大细胞胞吐过程中膜电容(Cm)的影响。结果水杨酸盐显著促进GTP-γ-S诱导的细胞膜厚度的增加，而氯丙嗪则显著抑制这种增加。水杨酸盐处理增加了染料在细胞表面的捕获量，而氯丙嗪处理则将其完全洗掉，表明两种药物都能引起肥大细胞膜表面的变形。结论本研究首次证实了膜双径，如水杨酸盐和氯丙嗪，可能相反地调节了肥大细胞的胞吐过程，这从cm的变化可以检测到。这些药物引起的质膜变形被认为是它们不同作用的原因。
Background/AimsSalicylate and chlorpromazine exert differential effects on the chemokine release from mast cells. Since these drugs are amphiphilic and preferentially partitioned into the lipid bilayers of the plasma membranes, they would induce some morphological changes in mast cells and thus affect the process of exocytosis.MethodsEmploying the standard patch-clamp whole-cell recording technique, we examined the effects of salicylate and chlorpromazine on the membrane capacitance (Cm) during exocytosis in rat peritoneal mast cells. Using confocal imaging of a water-soluble fluorescent dye, lucifer yellow, we also examined their effects on plasma membrane deformation of the cells.ResultsSalicylate dramatically accelerated the GTP-γ-S-induced increase in the Cm immediately after its application, whereas chlorpromazine significantly suppressed the increase. Treatment with salicylate increased the trapping of the dye on the cell surface, while treatment with chlorpromazine completely washed it out, indicating that both drugs induced membrane surface deformation in mast cells.ConclusionThis study demonstrated for the first time that membrane amphipaths, such as salicylate and chlorpromazine, may oppositely modulate the process of exocytosis in mast cells, as detected by the changes in the Cm. The plasma membrane deformation induced by the drugs was thought to be responsible for their differential effects.