Oscillatory Strain Promotes Vessel Stabilization and Alignment through Fibroblast YAP-Mediated Mechanosensitivity.
Oscillatory Strain Promotes Vessel Stabilization and Alignment through Fibroblast YAP-Mediated Mechanosensitivity.
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DOI：
10.1002/advs.201800506
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Levenberg S
中科院分区：
文献类型：
--
作者：
Landau S;Ben-Shaul S;Levenberg S
内皮细胞形成血管的内层，并且因此不断地暴露于剪切应力和机械应变。虽然剪切应力对血管生成的影响被广泛研究，但机械应变的作用却鲜为人知。为此，在振荡应变下共培养内皮细胞和成纤维细胞以产生血管网络。这两种细胞类型对机械刺激表现出明显不同的敏感性。成纤维细胞直接感受到应激，并通过增加排列、增殖、分化和迁移来响应，这由雅普易位到细胞核中促进。相反，内皮细胞通过跟踪成纤维细胞排列形成排列的血管。机械应变下结构中的雅普抑制会导致血管破坏，而在雅普抑制的静态对照中观察到的损伤较小。此外，机械刺激增强血管发育和稳定。此外，血管定向在植入小鼠背侧窗室中时被保留，并促进侵入的宿主血管以相同的方式定向。这项研究揭示了机械应变影响工程组织内血管发育的机制。这可以进一步用于设计适合于移植工程化移植物的更有组织和稳定的脉管系统。
Endothelial cells form the interior layer of blood vessels and, as such, are constantly exposed to shear stress and mechanical strain. While the impact of shear stress on angiogenesis is widely studied, the role of mechanical strain is less understood. To this end, endothelial cells and fibroblasts are cocultured under oscillatory strain to create a vessel network. The two cell types show distinctly different sensitivities to the mechanical stimulation. The fibroblasts, sense the stress directly, and respond by increased alignment, proliferation, differentiation, and migration, facilitated by YAP translocation into the nucleus. In contrast, the endothelial cells form aligned vessels by tracking fibroblast alignment. YAP inhibition in constructs under mechanical strain results in vessel destruction whereas less damage is observed in the YAP‐inhibited static control. Moreover, the mechanical stimulation enhances vessel development and stabilization. Additionally, vessel orientation is preserved upon implantation into a mouse dorsal window chamber and promotes the invading host vessels to orient in the same manner. This study sheds light on the mechanisms by which mechanical strain affects the development of blood vessels within engineered tissues. This can be further utilized to engineer a more organized and stable vasculature suitable for transplantation of engineered grafts.
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影响因子：
9.8
作者：
Chen Q;Jiang L;Li C;Hu D;Bu JW;Cai D;Du JL
通讯作者：
Du JL
DOI：
10.1016/j.bbamcr.2007.11.018
发表时间：
2008-03-01
影响因子：
5.1
作者：
Ernkvist, Mira;Birot, Olivier;Holmgren, Lars
通讯作者：
Holmgren, Lars
DOI：
10.1038/nrm3176
发表时间：
2011-08-23
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.2
作者：
Codelia, Veronica A.;Sun, Gongping;Irvine, Kenneth D.
通讯作者：
Irvine, Kenneth D.
影响因子：
3.1
作者：
IBA, T;SUMPIO, BE
通讯作者：
SUMPIO, BE