Enamel and dentin mineralization in familial hypophosphatemic rickets: a micro-CT study
Enamel and dentin mineralization in familial hypophosphatemic rickets: a micro-CT study
复制标题
DOI：
10.1259/dmfr.20140347
复制
发表时间：
2015-05-01
影响因子：
3.3
通讯作者：
Fonteles, C. S. R.
中科院分区：
文献类型：
--
作者：
Ribeiro, T. R.;Costa, F. W. G.;Fonteles, C. S. R.
目的：本研究的目的是使用microCT（piCT）分析X连锁低磷血症性佝偻病（XLHR）患者的牙釉质和牙本质矿化模式，并将乳牙和恒牙的牙釉质和牙本质矿化与牙齿位置、性别和有/无这种疾病相关联。方法：收集了来自同一家族的5个个体的19颗牙齿，其中1颗未受XLHR影响，4颗受XLHR影响。记录每名患者的性别、年龄、牙齿位置（前牙/后牙）和牙齿类型（乳牙/恒牙）。采集后，将牙齿置于0.1%百里酚溶液中，直至mu CT扫描。重建投影图像并进行分析。通过在存在釉质和牙本质的区域中平分每个牙齿的线来实现描述mu CT图像中的灰度距离关系的曲线图。测量并比较牙釉质和牙本质矿化密度。单变量方差分析和事后Tukey检验被用于所有comparation.Results：所有受影响的患者的牙齿提出了牙本质与不同的矿化模式相比，健康患者的牙齿与牙本质缺陷观察到旁边的牙髓腔。前牙和后牙的灰度值有显著性差异（p < 0.05），受累和未受累（P < 0.05），同时考虑体位和病情时，（p < 0.05）。总之，当比较有和没有胎心率的患者的牙齿时，牙本质的矿化模式不同，主要在牙髓腔附近，在那里发现具有多孔性的区域，因此发现较低的矿物密度和牙本质缺陷。
Objectives: The aim of the present study was to analyse the mineralization pattern of enamel and dentin in patients affected by X-linked hypophosphatemic rickets (XLHR) using microCT (piCT), and to associate enamel and dentin mineralization in primary and permanent teeth with tooth position, gender and the presence/absence of this disease.Methods: 19 teeth were collected from 5 individuals from the same family, 1 non-affected by XLHR and 4 affected by XLHR. Gender, age, tooth position (anterior/posterior) and tooth type (deciduous/permanent) were recorded for each patient. Following collection, teeth were placed in 0.1% thymol solution until mu CT scan. Projection images were reconstructed and analysed. A plot profile describing the greyscale distance relationship in mu CT images was achieved through a line bisecting each tooth in a region with the presence of enamel and dentin. The enamel and dentin mineralization densities were measured and compared. Univariate ANOVA and post hoc Tukey tests were used for all comparisons.Results: Teeth of all affected patients presented dentin with a different mineralization pattern compared with the teeth of healthy patients with dentin defects observed next to the pulp chambers. Highly significant differences were found for gray values between anterior and posterior teeth (p < 0.05), affected and non-affected (p < 0.05), as well as when position and disease status were considered (p < 0.05).Conclusions: In conclusion, the mineralization patterns of dentin differed when comparing teeth from patients with and without FHR, mainly next to pulp chambers where areas with porosity and consequently lower mineral density and dentin defects were found.