Inert phosphorescent nanospheres as markers for optical assays.
Inert phosphorescent nanospheres as markers for optical assays.
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惰性磷光纳米球作为光学测定的标记。
DOI：
10.1021/bc000130x
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发表时间：
2001
影响因子：
4.7
通讯作者：
O. Wolfbeis
中科院分区：
文献类型：
--
作者：
J. Kürner;I. Klimant;C. Krause;H. Preu;W. Kunz;O. Wolfbeis
提出了一种简单的封装技术，以产生适合发光标记的高磷光性惰性纳米球。它是基于磷光钌（II）-三（聚吡啶）配合物和聚丙烯腈（PAN）衍生物从N，N-二甲基甲酰胺溶液中的共沉淀。珠粒以非常小的球形聚集体的形式沉淀，典型的粒径小于50 nm。该方法允许将磷光和荧光染料包封在单独的纳米球中，条件是它们是足够亲脂的。由于使用PAN，氧气的淬火可以忽略不计。纳米球的特征在于其光谱特性（发光体的量子产率，亮度，发光衰减时间），在水性缓冲悬浮液中的稳定性，以及颗粒的大小，形状和表面电荷，以及储存稳定性，通过氧气淬灭，和染料浸出。
A simple encapsulation technique is presented to produce highly phosphorescent, inert nanospheres that are suitable luminescent markers. It is based on the coprecipitation of phosphorescent ruthenium(II)-tris(polypyridyl) complexes and polyacrylonitrile (PAN) derivatives from a solution in N,N-dimethylformamide. The beads precipitate in the form of very small aggregates of spherical shape and a typical particle diameter of less than 50 nm. This process allows the encapsulation of phosphorescent and fluorescent dyes in an individual nanosphere provided that they are sufficiently lipophilic. Quenching by oxygen is negligible due to the use of PAN. The nanospheres were characterized with respect to their spectral properties (quantum yields of the luminophores, brightness, luminescence decay time), stability in aqueous buffered suspensions, and in terms of size, shape, and surface charge of the particles, as well as storage stability, quenching by oxygen, and dye leaching.