Trophoblast invasion: Assessment of cellular models using gene expression signatures
Trophoblast invasion: Assessment of cellular models using gene expression signatures
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DOI：
10.1016/j.placenta.2010.08.011
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发表时间：
2010-11-01
期刊：
影响因子：
3.8
通讯作者：
Knoefler, M.
中科院分区：
文献类型：
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作者：
Bilban, M.;Tauber, S.;Knoefler, M.
人胎盘的侵袭性绒毛外滋养层细胞（EVT）与成功的妊娠结局密切相关，因为它们重塑子宫螺旋动脉以增加胎盘和发育中胎儿的血流和氧气输送。为了更深入地了解它们的生物学作用，通常使用不同的原代细胞培养模型。然而，进入早期胎盘组织可能是有限的，初级滋养层细胞在体外迅速停止增殖，损害遗传操作。因此，滋养层细胞系已被广泛用作研究EVT功能的替代物。虽然这些细胞系与它们的主要对应物共享一些分子标记，但它们在多大程度上再现EVT的侵袭表型尚不清楚。因此，我们在这里报告了SGHPL-5，HTR-8/SVneo，BeWo，JEG-3和新型ACH-3 P滋养层细胞与先前分析的原代绒毛细胞滋养层细胞（CTB）和绒毛外滋养层细胞（EVT）相比的第一次彻底的基因芯片分析。对约14，000种常见表达基因的分析显示，EVT最接近CTB，与绒毛膜癌细胞组（JEG-3、BeWo、ACH-3 P）和SV 40大T抗原选择的细胞类型组（SGHPL-5、HTR-8/SVneo）存在显著差异。类似地，对区分EVT与CTB的912个基因或370个EVT特异性基因的分析没有揭示出与任何原代细胞类型具有密切相似性的特定细胞系，尽管可以鉴定出EVT和每组细胞系共有的分子特征。考虑到mRNA表达模式的多样性，建议滋养层细胞系的分子研究需要在适当的主要模型系统中验证关键步骤。(C)2010爱思唯尔有限公司保留所有权利。
Invasive, extravillous trophoblasts (EVT) of the human placenta are critically involved in successful pregnancy outcome since they remodel the uterine spiral arteries to increase blood flow and oxygen delivery to the placenta and the developing fetus. To gain more insights into their biological role different primary cell culture models are commonly utilised. However, access to early placental tissue may be limited and primary trophoblasts rapidly cease proliferation in vitro impairing genetic manipulation. Hence, trophoblastic cell lines have been widely used as surrogates to study EVT function. Although the cell lines share some molecular markers with their primary counterpart, it is unknown to what extent they recapitulate the invasive phenotype of EVT. Therefore, we here report the first thorough GeneChip analyses of SGHPL-5, HTR-8/SVneo, BeWo, JEG-3 and the novel ACH-3P trophoblast cells in comparison to previously analysed primary villous cytotrophoblasts (CTBs) and extravillous trophoblasts (EVTs). Analyses of approximately 14,000 commonly expressed genes revealed that EVTs most closely resemble CTBs with considerable differences to the group of choriocarcinoma cells (JEG-3, BeWo, ACH-3P) and the group of SV40 Large T Antigen-selected cell types (SGHPL-5, HTR-8/SVneo). Similarly, analyses of 912 genes discriminating EVT from CTB, or 370 EVT-specific genes did not unravel a particular cell line with close similarity to any of the primary cell types, although molecular signatures common to EVT and each group of cell lines could be identified. Considering the diversity of mRNA expression patterns it is suggested that molecular studies in trophoblast cell lines require verification of the critical steps in an appropriate primary model system. (C) 2010 Elsevier Ltd. All rights reserved.