Self-assembling monolayer formation of glucose oxidase covalently attached on 11-aminoundecanethiol monolayers on gold
Self-assembling monolayer formation of glucose oxidase covalently attached on 11-aminoundecanethiol monolayers on gold
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DOI：
10.1039/b303298a
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发表时间：
2003-01-01
影响因子：
4.9
通讯作者：
Tazaki, M
中科院分区：
文献类型：
--
作者：
Nakano, K;Doi, K;Tazaki, M
利用 GOx 碳水化合物残基的化学氧化，将葡萄糖氧化酶 (GOx) 共价附着在 11-氨基十一烷硫醇 (AUDT) 自组装单层 (SAM) 上，形成均匀的酶单层，然后将所得“醛”酶与 SAM 中的末端氨基偶联，如 AFM 成像、IR、QCM 和电化学测量所表征。
Glucose oxidase (GOx) has been attached covalently to form uniform enzyme monolayers on self-assembled monolayers (SAMs) from 11-aminoundecanethiol (AUDT) by taking advantage of chemical oxidation of GOx carbohydrate residues followed by coupling the resulting 'aldehydic' enzyme with the terminal amino group in the SAM as characterized by AFM imaging, IR, QCM, and electrochemical measurements.