BIOCHEMISTRY AND MEASUREMENT OF FIBRINOGEN
BIOCHEMISTRY AND MEASUREMENT OF FIBRINOGEN
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DOI：
10.1093/eurheartj/16.suppl_a.6
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发表时间：
1995-03-01
影响因子：
39.3
通讯作者：
NIEUWENHUIZEN, W
中科院分区：
文献类型：
--
作者：
NIEUWENHUIZEN, W
纤维蛋白原是一个由三对不完全相同的多肽链组成的紧密相关的异质分子大家族：两个α-链、两个β-链和两个伽马链，通过二硫键连接在一起。纤维蛋白原的异质性是由于这三条链上的异质性。测定纤维蛋白原的方法主要有四种：凝血率法(克劳斯法)、可凝蛋白法、沉淀法和免疫学法。异质性可能因人而异，可能会影响不同检测方法中纤维蛋白原的表观浓度。一个更复杂的因素是，直到最近，还缺乏一个国际纤维蛋白原标准。高、低分子纤维蛋白原克劳斯：酶免疫测定法(EIA)比值在急性心肌梗死治疗过程中呈下降趋势，溶栓治疗后又回升至正常值以上。此外，高相对分子质量的纤维蛋白原比低相对分子质量的纤维蛋白原更容易凝结。这表明高分子量纤维蛋白原可能与血栓形成风险增加有关。仅通过功能分析(克劳斯)评估的纤维蛋白原与缺血性心脏病密切相关。虽然没有得到证实，但可以想象，在流行病学研究中，纤维蛋白原与类别：EIA比率为>；1的有更强的相关性。
Fibrinogen is a large heterogeneous family of closely related molecules consisting of three pairs of non-identical polypeptide chains: two Aalpha-, two Bbeta- and two gamma-chains, held together by disulphide bridges. The heterogeneity of fibrinogen is due to heterogeneities in all three chains. Four main types of assay are used to determine fibrinogen:clotting rate (Clauss), clottable protein, precipitation and immunological assays. Heterogeneities may differ from person to person and may affect the apparent fibrinogen concentrations in different assays. A further complicating factor was, until recently, the lack of an international fibrinogen standard. The ratio of Clauss:enzyme immunoassay (EIA) for high + low molecular weight fibrinogen decreases during therapy for acute myocardial infarction and increases again after thrombolytic therapy to above normal values. Furthermore, high molecular weight fibrinogen tends to clot more easily than low molecular weight fibrinogen. This suggests that high molecular weight fibrinogen might be associated with increased thrombotic risk. Fibrinogen assessed by a functional assay (Clauss) alone is strongly associated with ischaemic heart disease. Although not proven, it is conceivable that a fibrinogen with a Class:EIA ratio of >1 has an even stronger association in epidemiological studies.