The promiscuous evolutionary history of the family Bromoviridae
The promiscuous evolutionary history of the family Bromoviridae
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DOI：
10.1099/vir.0.2008/000166-0
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发表时间：
2008-07-01
影响因子：
3.8
通讯作者：
Elena, Santiago F.
中科院分区：
文献类型：
--
作者：
Codoner, Francisco M.;Elena, Santiago F.
重组和片段重组是RNA病毒长期遗传变异的重要因素，并经常参与新出现的病毒的发生。本研究探讨了这两个过程在植物病毒家族的进化辐射中所起的作用。以前已经用标准的分子系统发育方法探索了这个家族的进化史，但从不同的基因序列推断的树之间发现了不一致。如果RNA交换是一种常见的事件，这并不令人惊讶，因为众所周知，标准的系统发育方法很难描述基因组的重组和重新分类。为了协调这些差异，本研究首先探索了片段重组的程度，发现片段重组在雀麦病毒和黄瓜病毒的起源上是常见的，至少在紫花叶病毒、美国李线型病毒和柑橘叶皱纹病毒的起源上是常见的。其次，对这三个基因组RNA进行了重组分析，发现重组在布鲁姆病毒属、黄瓜病毒属和IlarVirus属的成员中非常普遍。还发现了几个涉及不同属物种的重组案例。最后，构建了反映这些遗传交流的系统发育网络。该网络确认了该科内不同属的分类地位，尽管遗传交换带来了系统发育方面的噪音。
Recombination and segment reassortment are important contributors to the standing genetic variation of RNA viruses and are often involved in the genesis of new, emerging viruses. This study explored the role played by these two processes in the evolutionary radiation of the plant virus family Bromoviridae. The evolutionary history of this family has been explored previously using standard molecular phylogenetic methods, but incongruences have been found among the trees inferred from different gene sequences. This would not be surprising if RNA exchange was a common event, as it is well known that recombination and reassortment of genomes are poorly described by standard phylogenetic methods. In an attempt to reconcile these discrepancies, this study first explored the extent of segment reassortment and found that it was common at the origin of the bromoviruses and cucumoviruses and at least at the origin of alfalfa mosaic virus, American plum line pattern virus and citrus leaf rugose virus. Secondly, recombination analyses were performed on each of the three genomic RNAs and it was found that recombination was very common in members of the genera Bromovirus, Cucumovirus and Ilarvirus. Several cases of recombination involving species from different genera were also identified. Finally, a phylogenetic network was constructed reflecting these genetic exchanges. The network confirmed the taxonomic status of the different genera within the family, despite the phylogenetic noise introduced by genetic exchange.