ISOLATION OF A FAST-GROWING STRAIN OF THE ROTIFER BRACHIONUS-CALYCIFLORUS PALLAS USING TURBIDOSTAT CULTURE
ISOLATION OF A FAST-GROWING STRAIN OF THE ROTIFER BRACHIONUS-CALYCIFLORUS PALLAS USING TURBIDOSTAT CULTURE
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DOI：
10.1016/0044-8486(88)90038-5
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发表时间：
1988-10-01
期刊：
影响因子：
4.5
通讯作者：
BORAAS, ME
中科院分区：
文献类型：
--
作者：
BENNETT, WN;BORAAS, ME
使轮虫萼花臂尾轮虫（Brachionus calyciflorus Pallus）在恒浊培养物中生长，此时迫使种群（克隆）以其最大比生长速率（μ max）生长14个月。在此期间，μ max从0.053/h（倍增时间= 13.1 h）增加51%至0.080/h（d.t. = 8.7 h），这是迄今报道的后生动物的最高生长率。当轮虫以低生长速率维持5个月（培养50代）时，快速生长的能力没有丧失，表明μ max升高是由于遗传组成或表达的变化。我们的程序增加人口的μ。最大值应适用于其他轮虫和后生动物物种，并有助于提高大规模生产的轮虫水产养殖的目的。
The rotifer Brachionus calyciflorus Pallus was grown in turbidostat culture, when the population (a clone) was forced to grow at its maximum specific growth rate (.mu.max) for a 14-month period. During this period, the .mu.max increased 51% from 0.053/h (doubling time = 13.1 h) to 0.080/h (d.t. = 8.7 h) after 8 months, the highest growth rate yet reported for a metazoan. The ability to grow rapidly was not lost when the rotifers were maintained at a low growth rate for 5 months (50 culture generations), indicating the elevated .mu.max was due to a change in genetic composition or expression. Our procedure for increasing a population''s .mu.max should be applicable to other rotifer and metazoan species, and be instrumental in increasing mass production of rotifers for aquacultural purposes.